Isolation and Selection of Indigenous Bacillus spp. Capable of Biodegradation of Organic Compounds from Artemia Ponds

Date Received: 25-08-2025

Date Accepted: 23-10-2025

Date Published: 31-10-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2025.23.10.09

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Issue: Vol. 23 No. 10 (2025): Tạp chí Khoa học Nông nghiệp Việt Nam

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Tam, L., Hai, V., Hoa, N., & Giang, H. (2025). Isolation and Selection of Indigenous Bacillus spp. Capable of Biodegradation of Organic Compounds from Artemia Ponds. Vietnam Journal of Agricultural Sciences, 23(10), 1364–1373. https://doi.org/10.31817/tckhnnvn.2025.23.10.09

Isolation and Selection of Indigenous Bacillus spp. Capable of Biodegradation of Organic Compounds from Artemia Ponds

Le Trung Tam (*) , Vu Hung Hai 1 , Nguyen Van Hoa 1 , Huynh Truong Giang 1

  • Tác giả liên hệ: [email protected]
  • 1 Trường Thủy sản, Đại học Cần Thơ

    • Keywords

    Bacillus, isolation, extracellular enzymes, Artemia culture ponds

    Abstract


    This study was conducted to isolate and screen Bacillus spp. as probiotic strains from water and sediment of Artemia culture systems. The research method includes sample collection, isolation, identification, preliminary screening and evaluation of Bacillus strains capable of producing extracellular enzymes, bacterial strain identification and safety assessment on Artemia. A total of 78 strains were isolated and characterized by morphological and physiological features similar to Bacillus genus by the conventional taxonomic method according to Bergey’s Manual (2015), which are rod-shaped cells, Gram-positive or negative, positive reactions to catalase and oxidase, motility and sporulation. Most isolates secreted extracellular enzymes (protease, amylase, and cellulase) capable of degrading casein, starch, and CMC, but 4 strains exhibited strong secretion of two types of extracellular enzymes by the spot-inoculated method. Notably, strain BC24.4 showed significantly higher protease and α-amylase activities (P<0.05) compared to the others. The result of the 16S rRNA gene sequencing showed that BC24.4 shared 99.3% similarity with Bacillus subtilis and safe on Artemia. Hence, strain BC24.4 is considered as potential for developing probiotic for enhancing degradation of organic matter in aquaculture systems, especially in Artemia culture ponds.

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