Isolation and Evaluation of the Lytic Ability of Bacteriophages on Pectobacterium spp. Causing Soft rot Disease of Chinese Cabbage (Brassica rapa subsp. pekinensis)

Date Received: 06-02-2025

Date Published: 20-02-2025

##submissions.doi##: https://doi.org/10.1234/kcpv8x29

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Issue: Vol. 22 No. 8 (2024)

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NÔNG HỌC

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Ha, V. T. N., & Han, T. T. N. (2025). Isolation and Evaluation of the Lytic Ability of Bacteriophages on Pectobacterium spp. Causing Soft rot Disease of Chinese Cabbage (Brassica rapa subsp. pekinensis). Vietnam Journal of Agricultural Sciences, 22(8). https://doi.org/10.1234/kcpv8x29

Isolation and Evaluation of the Lytic Ability of Bacteriophages on Pectobacterium spp. Causing Soft rot Disease of Chinese Cabbage (Brassica rapa subsp. pekinensis)

Vo Thi Ngoc Ha (*) , Truong Thi Ngoc Han

  • Tác giả liên hệ: [email protected]

    • Keywords

    Pectobacterium spp, lysis ability, plaque morphology, plaque diameters

    Abstract


    Soft rot disease, caused by Pectobacterium spp., is one of the most dangerous pathogens causing damage to numerous crops during production, harvesting, and storage. This study aimed to isolate and classify bacteriophages from Chinese cabbage-growing soil in Lam Dong and evaluate the parasitic and lytic properties of bacteriophage isolates on Pectobacterium spp. The results revealed that 14 bacteriophage isolates were identified and assigned to six groups. The plaque morphology was characterized by a big or small inner or opaque center surrounded by a translucent outer halo of varying diameters. All 14 bacteriophage isolates had a narrow host range and were capable of lysing Pectobacterium sp. isolate DT2. Two bacteriophage isolates FDR05 and FDR07 belonging to the Podoviridae family of the Caudovirales order with a polyhedral head and a short, non-contractile tail showed high bacterial lysis ability and plaque diameters were 13.58mm and 9.37mm, respectively.

    References

    Abbas S.D., Zahra E., Giti E. & Majid B. (2015). Isolation of Dickeya dadantii strains from potato disease and biocontrol by their bacteriophages. Brazilian Journal of Microbiology. 46(3): 791-797.

    Ackermann H.W. (2007). 5500 Phages examined in the electron microscope. Archives of virology.

    (2): 227-243.

    Bhat K.A., Masood S.D., Bhat N.A., Bhat M.A., Razvi S.M., Mir M.R., Sabina A., Wani N. & Habib M. (2010). Current status of post harvest soft rot in vegetables: A review. Asian Journal of Plant Sciences. 9(4): 200-208.

    Burgess L.W., Timothy E.K., Len T. & Phan Thúy Hiền (2009). Cẩm nang chuẩn đoán bệnh cây ở Việt Nam. Nhà xuất bản Trung tâm Nghiên cứu Nông nghiệp Quốc tế Australia (ACIAR). tr. 195.

    Buttimer C., Lucid A., Neve H., Franz C., O’mahony J., Turner D.J., Lavigne R. & Coffey A. (2018). Pectobacterium atrosepticum phage vB_PatP_CB5: a member of the proposed genus ‘Phimunavirus.’ Viruses. 26(10): 394.

    Czajkowski R., Pérombelon M.C.M., Jafra S., Lojkowska E., Potrykus M., van der Wolf J.M. & Sledz W. (2015). Detection, identification and differentiation of Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot: a review. Annals of Applied Biology. 166(1) : 18-38.

    Eayre C.G., Bartz J.A. & Concelmo D.E. (1995). The Bacteriophages of Erwinia carotovora and Erwinia ananas isolated from freshwater lakes. Plant Disease. 79: 801-804.

    Fanaei P.R., Wagemans J., Kunisch F., Lavigne R., Trampuz A. & Gonzalez M. M. (2022). Novel Stenotrophomonas maltophilia bacteriophage as potential therapeutic agent. Pharmaceutics.

    (10): 2216.

    Glasner J.D., Marquez-Villavicencio M., Kim H.S., Jahn C.E., Ma B., Biehl B.S., Rissman A.I., Mole B., Yi X., Yang C.H., Dang J.L., Grant S.R., Perna N.T. & Charkowski A.O. (2008). Niche-specificity and the variable fraction of the Pectobacterium pan-genome. Molecular Plant-Microbe Interacttion. 21:1549-1560.

    Golkhandan E., Kamaruzaman S., Sariah M., Abidin M. A., Nazerian E. & Yassoralipour A. (2013). First Report of Soft Rot Disease Caused by Pectobacterium wasabiae on Sweet Potato, Tomato, and Eggplant in Malaysia. Plant Disease. 97(5): 685

    Gross D.C., Powelson M.L., Regner K.M., & Radamaker G.K. (1991). A bacteriophage-typing system for surveying the diversity and distribution of strains of Erwinia carotovora in potato fields. Phytopathology. 81: 220-226.

    Jurczak-Kurek A., Gąsior T., Nejman-Faleńczyk B., Bloch S., Dydecka A., Topka G., Necel A., Jakubowska-Deredas M., Narajczyk M., Richert M., Mieszkowska A., Wróbel B., Węgrzyn G. & Węgrzyn A. (2016). Biodiversity of bacteriophages: morphological and biological properties of a large group of phages isolated from urban sewage. Scientific Reports. 6: 34338.

    Kabanova A., Shneider M. M., Bugaeva E., Ha V.T.N., Miroshnikov K.A., Korzhenkov A., Kulikov E.E., Toschakov S., Ignatov A. & Miroshnikov K.A. (2018). Genomic characteristics of vB_PpaP_PP74, a T7-like Autographivirinae bacteriophage infecting a potato pathogen of the newly proposed species Pectobacterium parmentieri. Advanced Virology. 163:1691-1694.

    Kim D., Kim N., Kim C., Jeong M.I., Oh K.K., Kim B.E., Ryu J.G., Jung J., Jee S., Ryu K.Y. (2021). Investigation of antimicrobial minimum inhibitory concentration of Pectobacterium spp. isolated from agricultural produce. The Korean Journal of Pesticide Science. 25: 333-342.

    Kim H.S., Park Y.H., Nam H.; Lee Y.M., Song K., Choi C., Ahn I., Park S.R., Lee Y.H., Hwang D.J. & Van Wees S. (2014). Overexpression of the Brassica rapa transcription factor WRKY12 results in reduced soft rot symptoms caused by Pectobacterium carotovorum in Arabidopsis and Chinese cabbage. Plant Biology. 16(5): 973-981.

    Kropinski A.M., Mazzocco A., Waddell T.E., Lingohr E. & Johnson R.P. (2009). Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay. In: Clokie M.R., Kropinski A.M. (eds) Bacteriophages. Methods in Molecular Biology™. Humana Press. Vol. 501.

    Lai M.J., Chang K.C., Huang S.W., Luo C.H., Chiou P.Y., Wu C.C. & Lin N.T. (2016). The tail associated protein of Acinetobacter baumannii phage ÖAB6 is the host specificity determinant possessing exopolysaccharide depolymerase activity. PloS One. 11(4): e0153361.

    Lee S.Y., Magar R.T., Kim H.J., Choi K. & Lee S.W. (2021). Complete genome sequence of a novel bacteriophage RpY1 infecting Ralstonia solanacearum strains. Current Microbiology. 78: 2044-2050.

    Lim J.A., Jee S., Lee D.H., Roh E., Jung K., Oh C. & Heu S. (2013). Biocontrol of Pectobacterium carotovorum subsp. carotovorum using bacteriophage PP1. Journal of Microbiol Biotechnology. 23(8): 1147-1153.

    Lương Hữu Tâm & Nguyễn Thị Thu Nga (2014). Bước đầu phân lập và đánh giá khả năng ký sinh, tính đặc hiệu của một số chủng thực khuẩn thể của vi khuẩn Xanthomonas oryzae pv. oryzae. Hội thảo quốc gia Bệnh hại thực vật. 13: 76-84.

    Mansfield J., Genin S., Magori S., Citovsky V., Sriariyanum M., Ronald P., Dow M., Verdier V., Beer S.V., Machado M.A., Toth I., Salmond G. & Foster D.R. (2012). Top 10 plant pathogenic bacteria in molecular plant pathology. Molecular plant pathology. 13(6): 614-629.

    Murphy A. Frederick, Claude M. Fauquet, Bishop D.H., Ghabrial S.A., Jarvis A.W., Martelli G.P., Mayo M.A. & Summers M.D. (1995). Virus Taxonomy : Classification and Nomenclature of Viruses, Sixth Report of the International Committee on Taxonomy of Viruses. Springer.

    Nguyen T.V., Roh E., Nguyen T.T. & Oh C.S. (2022). Antibiotic Resistance of Pectobacterium Korean Strains Susceptible to the Bacteriophage phiPccP-1. Res. Plant Disease. 28(3): 166-171.

    Nguyễn Thị Trúc Giang, Nguyễn Thị Thu Nga & Đoàn Thị Kiều Tiên (2014). Phân lập thực khuẩn thể và đánh giá hiệu quả phòng trị bệnh cháy bìa lá lúa do vi khuẩn Xanthomonas oryzae pv. oryzae. Tạp chí Khoa học Đại học Cần Thơ. (Chuyên đề Nông nghiệp): 194-203.

    Nguyễn Thúy An, Nguyễn Thị Thu Nga, Phạm Văn Kim và Nguyễn Văn Minh Phụng (2017). Phân lập và tuyển chọn các dòng thực khuẩn thể trong phòng trừ bệnh héo xanh trên cây hoa vạn thọ (Tagetes papula L.) do vi khuẩn Ralstonia solanacearum Smith. Tạp chí Khoa học Trường Đại học Cần Thơ. 49b: 44-52

    Phan Quốc Huy, Hồ Cãnh Thịnh, Nguyễn Thị Thu Nga & Phạm Nguyễn Minh Trung (2016). Đánh giá hiệu quả của thực khuẩn thể trong phòng trừ bệnh thối hạt trên lúa do vi khuẩn Burkholderia glumae. Tạp chí Khoa học Trường Đại học Cần Thơ.

    b: 70-78.

    Ravensdale M., Blom T.J., Gracia-Garza J.A., Svircev A.M. & Smith R.J. (2007). Bacteriophages and the control of Erwinia carotovora subsp. carotovora. Canadian Journal of Plant Pathology. 29: 121-130.

    Roger Shivas & Dean Beasley (2005). Management of plant pathogen collections. Diparment of Agriculture Fisheries Forestry Australia Government.

    Romero-Calle D., Guimarães B.R., Góes-Neto A. & Billington C. (2019). Bacteriophages as alternatives to antibiotics in clinical care. Antibiotic (Basel). 8 : 138.

    Sabour P.M. & Griffiths M.W. (2010). Bacteriophages in the control of foodand waterborne pathogens. American Society for Microbiology Press.

    Toth I.K., Bell K.S., Holeva M.C. & Birch P.R.J. (2003). Soft rot erwiniae: from genes to genomes. Molecular Plant Pathology. 4(1): 17-30. doi:10.1046/j.1364-3703.2003.00149.

    Weinbauer M.G. (2004). Ecology of prokaryotic viruses. FEMS Microbiology Reviews. 28: 127-81.

    Wommack K.E. & Colwell R.R. (2000). Virioplankton: viruses in aquatic ecosystems. Microbiology and Molecular Biology. Review. 64: 69-114.

    Xie H., Li X.Y., Ma Y.L. & Tian Y. (2017). First report of Pectobacterium aroidearum causing soft rot of Chinese cabbage in China. Plant Disease, PDIS-07-