Date Received: 06-06-2025
Date Published: 13-06-2025
##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2013.11.6.
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How to Cite:
Ovary Slice Culture Technique for Embyro Rescue of Interspecific Crosses between Lilium longiflorum and L. brownii var. colchesteri
,
Hiroshi Okubo
Keywords
Interspecific cross, L. brownii var. colchesteri, L. longiflorum
Abstract
Lilium brownii var. colchesteri (BRO) has an unique characteristic, i.e., the flower color changes from yellowish-cream to white during anthesis. For introducing this trait to cut flower production in Lilium, interspecific crosses of L. longiflorum (as seed parents) and BRO (as a pollen parent) were carried out. Capsules with unviable seeds were found in all crosses. To overcome the incongruity barrier, ovary slices excised from developing ovaries at 7 to 28 days after pollination (DAP) were cultured in a Murashige and Skoog medium supplemented with 40g·L-1 sucrose, 40g·L-1 D-mannitol and 2.5g·L-1 gellan gum under 24h illumination. The hybridity of germinated bulblets were confirmed before acclimatization by isozyme analysis. Hybrid plantlets were obtained from the crosses of L. longiflorum ‘LPI’ × BRO. After acclimatization and cultivation, 11 hybrid plants produced flowers changing from yellowish cream to white during anthesis as does the pollen parent.
References
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