Date Received: 06-02-2025 / Date Published: 20-02-2025
Soft rot disease, caused by Pectobacterium spp., is one of the most dangerous pathogens causing damage to numerous crops during production, harvesting, and storage. This study aimed to isolate and classify bacteriophages from Chinese cabbage-growing soil in Lam Dong and evaluate the parasitic and lytic properties of bacteriophage isolates on Pectobacterium spp. The results revealed that 14 bacteriophage isolates were identified and assigned to six groups. The plaque morphology was characterized by a big or small inner or opaque center surrounded by a translucent outer halo of varying diameters. All 14 bacteriophage isolates had a narrow host range and were capable of lysing Pectobacterium sp. isolate DT2. Two bacteriophage isolates FDR05 and FDR07 belonging to the Podoviridae family of the Caudovirales order with a polyhedral head and a short, non-contractile tail showed high bacterial lysis ability and plaque diameters were 13.58mm and 9.37mm, respectively.
