Study on The Transfer of Auxin Susceptible Gene (Ino-Rolb) into Duong Canh Mandarin and Vinh Orange

Date Received: 07-06-2025

Date Published: 13-06-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2013.11.6.

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Issue: Vol. 11 No. 6 (2013)

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NÔNG HỌC

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Ton, P., Hai, T., Lu, D., Bach, N., & Dandenkar, A. . (2025). Study on The Transfer of Auxin Susceptible Gene (Ino-Rolb) into Duong Canh Mandarin and Vinh Orange. Vietnam Journal of Agricultural Sciences, 11(6). https://doi.org/10.31817/tckhnnvn.2013.11.6.

Study on The Transfer of Auxin Susceptible Gene (Ino-Rolb) into Duong Canh Mandarin and Vinh Orange

Phan Huu Ton (*) , Tong Van Hai , Doan Van Lu , Nguyen Duc Bach , Abhaya Dandenkar

  • Tác giả liên hệ: [email protected]

    • Keywords

    Acetosyringone, Agrobacterium tumerfacies, auxin-sensitive gene (INO-rolB), co-culture, gene transformation

    Abstract


    The apomixis phenomenon in citrus often leads to small, seedless and poor quality fruits. It is due to no or very less auxin is synthesized in the ovary leading to the abnormal development of embryo. Therefore, the fruits can not develop normally. Of many different strategies for seedless breeding in citrus such as outcrossing, triploidy, mutagenesis, the transfer of auxin-sensitive gene particularly expressed in ovary is a very useful approach. In this study, the vector pDU04.4522 carrying the auxin-sensitive gene (INO-rolB) working under the control of INO promoter was transferred into Duong Canh mandarine and Vinh orange. In order to identify the optimal conditions for gene transfer, four experiments focusing on the co-culture stage were carried out. Results showed that the auxin-sensitive gene INO-rolB was sucessfully transferred into the cotyledon of  Duong Canh tangerine and Vinh orange. The optimal conditions for  high efficiency of gene transfer in co-culture medium were at 22°C for 3 days in the presence of 50 - 100mM acetosyringone and 2 mg/l NAA.

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