Characterization of Yeast Isolated from Rice Wine Starter Cakes in Mekong Delta

Date Received: 06-06-2025

Date Published: 13-06-2025

Views

0

Downloads

0

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Thanh, N., Duyen, N., Lam, B., & Trach, N. (2025). Characterization of Yeast Isolated from Rice Wine Starter Cakes in Mekong Delta. Vietnam Journal of Agricultural Sciences, 10(2). https://doi.org/10.31817/tckhnnvn.2012.10.2.

Characterization of Yeast Isolated from Rice Wine Starter Cakes in Mekong Delta

Nguyen Huu Thanh (*) , Nguyen Thi Ky Duyen , Bang Hong Lam , Nguyen Quang Trach

  • Tác giả liên hệ: [email protected]
  • Keywords

    Saccharomyces cerevisiae, starter cakes, thermo-resistant, alcohol tolerance

    Abstract


    As a source of inoculation starters in the manufacture of alcohol from rice varieties from the Mekong River Delta, Vietnam, Banh men has been produced since the ancient time. These starters, which normally combine three groups of microorganisms, viz. yeasts, bacteria and moulds convert the starchy materials into fermentable sugar and subsequently to alcohol and organic acids. Yeasts are significant in the production of traditional beverage because they play the main role in alcoholic fermentation. Of 128 strains of yeasts isolated from rice fermenting staters in the Mekong River delta, 30 yeast strains were identified to be thermo-resistant at 50oC and  ethanol tolerance at 17% (v/v) in the challenge test with added ethanol with good flocculation and sporulation. From characterization of 10 yeast strains, 7 yeasts strains were identified as Saccharomyces cerevisiae and 3 others as Clavispora lusitaniae.

    References

    Amoore, J.E and E. Hautala (1983). Odor as an aid to chemical safety: odor thresholds compared with threshold limit values and volatilities for 214 industrial chemicals in air and water dilution. Journal of Applied Toxicology 3, 272-290.

    Đồng Thị Thanh Thu (2003). Sinh hóa ứng dụng, TP HCM, NXB Đại học Quốc gia.

    Guimarães Thais M., G. Moriel Danilo, P. Machado Iara, M.T. Cyntia, Fadel Picheth, M. Tania and B. Bonfim (2006). Isolation and characterization of Saccharomyces cerevisiae strains of winery interest. Brazilian Journal of Pharmaceutical Sciences vol. 42. n. 1. Jan. /Mar.

    Ngô Thị Phương Dung, Rombouts and Nout (2005). Development of defined mixed-culture fungal fermentation starter granulates for controlled production of rice wine. Innovative Food Science & Emerging Technologies, Volume 6, Issue 4, 1 December 2005, Pages 429-441.

    Oliveira VA, M.A.Vicente, L.G. Fietto, I.M. Castro, M.X. Coutrim, D. Schüller, R.L.Brandão and al (2008). Biochemical and Molecular Characterization of Saccharomyces cerevisiae strains Obtained from Sugar-Cane Juice Fermentations and Their impact in Cachaca Production. Appl. Environ. Microbiol, vol. 74. p. 3693-3701.

    Ono, B. I, N. Ishi, S. Fujino, I. Aoyama (1991). I. Role ofhydrosulfide ions (HS-) in methylmercury resistance in Saccharomyces cerevisiae. Appl. Environ. Microbiol.v. 57, p. 3183-3186.

    Ralser (2010). Quick and Easy Isolation of Genomic DNA from Yeast. Acess online ngày 17/10/2010. http://www.protocol-online.org/prot/Protocols/Quick-and-Easy-Isolation-of-Genomic-DNA-from-Yeast-3451.html.

    Sandhu, G.S., B.C. Kline, L.Stockman and G.D. Roberts (1995). Molecular probes for diagnosis of fungal infections. J. Clin. Microbiol. 33:2913-2919.

    Vu Nguyen Thanh, Le Thuy Mai and Duong Anh Tuan (2008). Microbial diversity of traditional Vietnamese alcohol fermentation starters (banh men) as determined by PCR-mediated DGGE. International Journal of Food Microbiology. Volume 128, Issue 2, 10 December 2008, Pages 268-273.