Co-expression of Human Interleukin-2 Gene with Chaperone GroESL and Thioredoxin in Escherichia coli BL21 (DE3)

Date Received: 06-06-2025

Date Published: 13-06-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2013.11.6.

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Issue: Vol. 11 No. 6 (2013)

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Anh, L., Anh, L., Khoa, D., Hong, L., Nguyet, P., Duc, N., … Hai, T. (2025). Co-expression of Human Interleukin-2 Gene with Chaperone GroESL and Thioredoxin in Escherichia coli BL21 (DE3). Vietnam Journal of Agricultural Sciences, 11(6). https://doi.org/10.31817/tckhnnvn.2013.11.6.

Co-expression of Human Interleukin-2 Gene with Chaperone GroESL and Thioredoxin in Escherichia coli BL21 (DE3)

Le Thi Lan Anh (*) , Le Phuong Hoang Anh , Dao Trong Khoa , Le Thi Thu Hong , Phung Thu Nguyet , Nguyen Huu Duc , Nguyen Thi Hang , Truong Nam Hai

  • Tác giả liên hệ: [email protected]

    • Keywords

    Chaperone, expression, interleukin-2, thioredoxin, pelB secret signal

    Abstract


    Interleukin 2 (IL-2), one of the cytokines plays an important role for the immune response. IL-2 promotes the activation of T cells and so often used in immunotherapy such as kidney epithelial malignant and melanoma cancer. Gene encoding il-2 modified Cys125 to Ser was inserted into pET22b(+) harboring pelB secrete signal for expression in E. coli BL21(DE3) cells. To enhance the correct protein folding and solubility, IL-2 was simultaneously expressed with chaperone GroESL and thioredoxin (Trx). The results indicated that IL-2 proteins co-expressed with GroESL and Trx of 20 kDa were still existed in insoluble form and consisted of the pelB secret signal.  

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