Construction and Development of Yeast-based Biosensor Applied for Detection of Genotoxic Chemical Compounds

Date Received: 06-06-2025

Date Published: 13-06-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2013.11.6.

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Issue: Vol. 11 No. 6 (2013)

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NÔNG HỌC

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Ngoc, B., Duc, N., Thang, N., & Hieu, N. (2025). Construction and Development of Yeast-based Biosensor Applied for Detection of Genotoxic Chemical Compounds. Vietnam Journal of Agricultural Sciences, 11(6). https://doi.org/10.31817/tckhnnvn.2013.11.6.

Construction and Development of Yeast-based Biosensor Applied for Detection of Genotoxic Chemical Compounds

Bui Van Ngoc (*) , Nguyen Huu Duc , Nguyen Duc Thang , Nguyen Cong Hieu

  • Tác giả liên hệ: [email protected]

    • Keywords

    Biosensor, cytotoxicity, genotoxicity, GFP, methyl methansulfonate, menadione, reporter gene

    Abstract


    Biosensor was developed by design and construction of a reporter plasmid containing GFP gen under regulation of RNR2 promoter. This reporter plasmid was transformed in yeast cells which were then treated with different concentrations of MMS and menadione. Due to induction of genotoxic compounds, such as MMS, RNR2 promoter regulates transcription and translation of GFP leading to fluorescence signal measured at 485nm (excitation wave length) and 535nm (emission wave length). Fluorescence instensity was directly proportional to MMS concentrations when yeast cells treated with less than 1mM in the environment, but decreasing when exposed to higher MMS concentrations as a result of inhibition of cellular respiration and ATP synthesis. In contrast, promoter RNR2 was not induced by menadione resulting in no fluorescence signal. Thus, this kind of biosensor is able to not only determine and detect genotoxic chemical compounds, which are harmful to human and environment, but also identify new and uncharacterized compounds which possess the same chemical properties as MMS, and could be used in new drug discovery and drug screening.

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