Genetic Association between Single Nucleotide Polymorphisms of PIT-1 Gene and Milk Production in Holstein Friesian Cows

Date Received: 07-06-2025

Date Published: 13-06-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2013.11.6.

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Issue: Vol. 11 No. 6 (2013)

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Thu, N., Hang, N., Duc, N., Tùng, H., Chau, L., Ty, L., & Thuy, N. (2025). Genetic Association between Single Nucleotide Polymorphisms of PIT-1 Gene and Milk Production in Holstein Friesian Cows. Vietnam Journal of Agricultural Sciences, 11(6). https://doi.org/10.31817/tckhnnvn.2013.11.6.

Genetic Association between Single Nucleotide Polymorphisms of PIT-1 Gene and Milk Production in Holstein Friesian Cows

Nguyen Thi Thu , Nguyen Thi Minh Hang , Nguyen Huu Duc , Ha Thanh Tùng , Le Thi Chau , Le Van Ty , Nguyen Thi Dieu Thuy (*)

  • Tác giả liên hệ: [email protected]

    • Keywords

    Genetic correlation, Holstein cows, milk yield, Lactation length, PIT-1 gene

    Abstract


    PIT-1, a regulatory transcription factor, plays a role in pituitary cell differentiation and proliferation and expression level of hormones in the anterior pituitary gland. The objective of present study was to identify the single nucleotide polymorphisms of PIT-1 gene and their association with milk yield in Holstein Friesian (HF) cows. Milk yield was recorded for the whole lactation period. PCR-RFLP technique was applied for genotyping of two SNPs belonging to exon 2 (E2) and exon 6 (E6) with restriction enzymes TaqI and HinfI. The analysis of 53 Holstein milking cows showed that genotypic frequencies of AA, AB and BB of PIT-1E2 were 45.3, 28.3 and 26.4%, respectively. For the PIT-1E6, the highest frequency of GG genotype (73.6%), the lowest frequency of genotype TT (7.5%) and TG genotype (18.9%) were found. Minitab software was used to analyze genotype - phenotype association. The result revealed that theere exists positive association between locus PIT-1E2 with milk yield (P= 0.037 and ρ = 0.28) and number of milking day (P=0.015 and r= 0.306), whereas, no association of PIT-1E6 was found (P>0.05). Cows with AA genotype yielded more milk than those with BB and AB genotypes. These finding showed the potential use of AA genotype of PIT-1E2 in assisting breeding for increased milk production of Holstein Friesian cows.

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