Protocol for Indentification in Tra Catfish (Pangasianodon hypophthalmus) by Morphological Taxonomyand Molecular Genetics methods

Date Received: 02-07-2025

Date Published: 03-07-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2017.15.7.

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Issue: Vol. 15 No. 7 (2017)

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Ha, T., Hang, T., Huong, N., & Thuy, N. (2025). Protocol for Indentification in Tra Catfish (Pangasianodon hypophthalmus) by Morphological Taxonomyand Molecular Genetics methods. Vietnam Journal of Agricultural Sciences, 15(7). https://doi.org/10.31817/tckhnnvn.2017.15.7.

Protocol for Indentification in Tra Catfish (Pangasianodon hypophthalmus) by Morphological Taxonomyand Molecular Genetics methods

Tran Thi Thuy Ha (*) , Tran Nguyen Ai Hang , Nguyen Thi Huong , Nguyen Thi Thu Thuy

  • Tác giả liên hệ: [email protected]

    • Keywords

    COI, Cyt b, identification, morphology, sequence, Pangasianodon hypophthalmus

    Abstract


    The combination of morphological taxonomy and molecular genetics methods is required for effective and accurate identification of Tra catfish (Pangasianodon hypophthalmus). The identification based on morphological characteristics is possible to identify accuratelyPangasiidae species that simply relieson number of spines, pelvic fins, pectoral fins, etc. In addition, sequence analysis of cytochrome c oxidase I (COI) andcytochrome b (Cytb) genes along with specialized softwares provide significantly reliable results, which contribute information to confirm the morphological classification. In this study, idetification based on molecular genetics method revealed 99-100% similarity in nucleotide sequences of COI and Cytb genes between 30 fish samples collected in An Giang and Ben Tre provinces with Tra catfish Pangasianodon hypophthalmus species. Our finding confirms the superiority and necessity of combining two identifications methods, morphological taxonomy and molecular genetics methods.

    References

    Azlina, Z.M., Daud, S.K., Siraj, S.S., Aliabadian M. and Moghaddam, F.Y. (2013). Molecular phylogeny of Malaysian Pangasiid based on cytochrome C oxidase I (COI) DNA sequences.

    Docker, M.F. and Heath, D.D. (2003). Genetic comparison between sympatric anadromous steelhead and freshwater resident rainbow trout in British Columbia, Canada. Conservation Genetics, 4: 227-231.

    Eschmeyer W.N. (1998). Catalog of Fishes - California Academy of Sciences, USA.

    Fishbase. Truy cập ngày 5/7/2015 tại www. Fishbase.org.

    Hebert, P.D.N., Cywinska, A., Ball, S.L. and deWaard, J.R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences, 270: 313-321.

    Hebert, P.D.N., Ratnasingham, S. and de Waard, J.R. (2004). “Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species”, Proceeding of the Royal Society Part B: Biological Sciences, 270(1): 96 - 99.

    Hellberg, R.S. and Morrissey M.T. (2011). Advances in ADN-based techniques for the detection of seafood species substitution on the commercial market Part B: Biological Sciences, 270(1512): 313-322.

    Hubert, N., Hanner, R., Holm, E., Mandrak, N.E., Taylor, E., Burridge, M., Watkinson, D., Dumont, P., Curry, A., Bentzen, P., Zhang, J., April, J. and Bernatchez, L. (2008). Identifying Canadian freshwater fishes through DNA barcodes. Plos ONE 3e2490 -2490.

    Kartavtsev, Y.P. and Lee, J.S. (2006). Analysis of nucleotide diversity at genes Cyt-b and Co-1on population, species and genera levels. Applicability of DNA and allozyme data in genetics of speciation. Russ. J. Genet., 42: 341-362.

    Mai Đình Yên, Nguyễn Văn Trọng, Nguyễn Văn Thiện, Lê Hoàng Yến và Hứu Bạch Loan (1992). Định loại cá nước ngọt Nam bộ. Nhà xuất bản Khoa học và Kỹ thuật, Hà Nội, 343 trang.

    Marko, P.B., Lee, S.C., Rice, A.M., Gramling, J.M., Fitzhenry, T.M., McAlister. J.S., Harper, G.R. and Moran, A.L. (2004). Fisheries: Mislabeling of a depleted reef fish. Nature, 430: 309-310.

    Nguyễn Đức Bách, Nguyễn Minh Khuyến, Ngô Thanh Hương và Nguyễn Thị Mai (2016). Phân loại cá chim vây vàng bằng đánh giá hình thái và phân tích trình tự gen ti thể MT-COI. Báo cáo khoa học về nghiên cứu và giảng dạy sinh học ở Việt Nam, Hội nghị khoa học toàn quốc lần thứ hai, Đà Nẵng.

    Nguyễn Phương Thảo và Dương Thúy Yên (2015). So sánh đặc điểm hình thái và DNA mã vạch của hai loài cá bống trân Butis butis và Butis humeralis. Tạp chí khoa học, Đại học Cần Thơ, 40: 23-30.

    Nguyễn Văn Hảo (2005). Cá nước ngọt Việt Nam, tập II, Nhà xuất bản Nông nghiệp, Hà Nội.

    Norton, S.F., Luczkovich, J.J. and Motta, P.J. (1995). The role of ecomorphological studies in the comparative biology of fishes. Environment. Biology. Fish, 44: 287-304.

    Pouyaud, L., Teugels, G.G., Gustiano, R. and Legendre, M. (2000). Contribution to the phylogeny of pangasiid catfishes based on allozymes and mitochondrial DNA. Journal of Fish Biology, 56: 1509-1538.

    Quyen, H.D.V., Phuong, T.T.L., Oanh, T.T., Thuoc, T.L. and Binh, D.T. (2015). Phylogenetic relationships of of Freshwater Fish in Vietnamese Mekong. International conference on biological, environment and food engineering (BEFE), Singapore, May 15-16, 2015.

    Rainboth W.J. (1996). Fishes of the Cambodian Mekong FAO.

    So Nam, Eric Baran and Leng Sy Vann (2011). Identification key to Pangasiid catfishes in Cambodia.

    Steinke, D., Zemlak, T.S., Boutillier, J.A. and Hebert, P.D.N. (2009). DNA barcoding of Pacific Canada’s fishes. Mar Biol., 156: 2641-2647.

    Tamura, K., Stecher G., Peterson D., Filipski A. and Kumar S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30: 2725-2729.

    Wainwright, P.C. (1996). Ecological explanation through functional morphology: the feeding biology of sunfishes. Ecology, 77: 1336-1343.

    Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R. and Hebert, B.D.N. (2005). DNA barcoding Australia’s fish species. Philosophical Transactions of The Royal Society B: Biological Scienses, 360: 1847-1857.

    Wang M., Yang J.X. and Chen X.Y. (2013). “Molecular phylogeny and biogeography of percocypris (cyprinidae, teleostei)”, PloS One 8, e61827.

    Wolf, C., Burgener, M., Hübner, P. and Lüthy, J. (2000). PCR-RFLP analysis of mitochondrial DNA: differentiation of fish species. LWT-Food Science and Technology, 33: 144-150.

    Xu, H., Zhang, Y., Xu, D., Lou, B., Guo, Y., Sun, X. and Guo, B. (2014). Genetic population structure of miiuy croaker (Miichthys miiuy) in the Yellow and East China Seas base on mitochondrial COI sequences. Biochemical Systematics and Ecology, 54: 240-246.