Induction and Culture of Adventitious Peatnut Roots as a Source of Resveratrol

Date Received: 04-07-2025

Date Published: 04-07-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2022.20.11.

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Issue: Vol. 20 No. 11 (2022)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Son, D., Huyen, C., Hanh, N., & Thao, N. (2025). Induction and Culture of Adventitious Peatnut Roots as a Source of Resveratrol. Vietnam Journal of Agricultural Sciences, 20(11). https://doi.org/10.31817/tckhnnvn.2022.20.11.

Induction and Culture of Adventitious Peatnut Roots as a Source of Resveratrol

Dinh Truong Son , Chu Thi Thu Huyen , Nguyen Thi Hanh , Ninh Thi Thao (*)

  • Tác giả liên hệ: [email protected]

    • Keywords

    Adventitious roots, auxin, peanuts, resveratrol, sucrose

    Abstract


    Resveratrol as a phytochemical with strong antioxidant properties was found in certain berries and in peatnut roots. This study was conducted to induce adventitious roots of peatnut and investigate some factors affecting the growth of adventitious roots as a source of resveratrol. Leaf explants were cultured on MS medium supplemented with α-NAA, IBA and IAA (1-10 mg/l), sucrose (20-50 g/l) under light and dark culture conditions to induce formation of adventitious roots. The results showed that direct formation of adventitious roots from peanut leaves was best on MS medium containing 5 mg/l α-NAA and 30 g/l sucrose cultured in full darkness with 87.5% rooting rate and 5.5 roots per explant after 6 weeks of culture. The growth of adventitious roots (1.0cm in length) when cultured on MS medium sustained better than ½ MS, B5 and ½ B5 media. A positive effect of auxin including α-NAA, IBA and IAA on adventitious root growth was found. The maxium percentage of adventitious root segment producing lateral roots and highest number of lateral roots/segment, (100% and 4.9 roots, respectively) were obtained on MS + 1 mg/l α-NAA after 6 weeks of culture.

    References

    Alallaq S., Ranjan A., Brunoni F., Novák O., Lakehal A. & Bellini C. (2020). Red light controls adventitious root regeneration by modulating hormone homeostasis in Picea abies seedlings. Frontiers in Plant Science. 11: 586140.

    Cui X.H., Murthy H.N., Wu C.H. & Paek K.Y. (2010). Adventitious root suspension cultures of Hypericum perforatum: effect of nitrogen source on production of biomass and secondary metabolites. In vitro culture and Developmental Biology Plant. 46(5): 437-444.

    Gamborg O.L., Miller R.A. & Ojima K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research. 50(1): 151-158.

    Gligorijević N., Stanić-Vučinić D., Radomirović M., Stojadinović M., Khulal U., Nedić O. & Ćirković Veličković T. (2021). Role of resveratrol in prevention and control of cardiovascular disorders and cardiovascular complications related to COVID-19 disease: Mode of action and approaches explored to increase its bioavailability. Molecules. 26: 2834.

    Hussain M.J., Abbas Y., Nazli N., Fatima S., Drouet S., Hano C. & Abbasi B.H. (2022). Root cultures, a boon for the production of valuable compounds:

    A comparative review. Plants. 11: 439.

    Hussein S., Ling A.P.K., Ng T.H., Ibrahim R. & Paek K.Y. (2012). Adventitious roots induction of recalcitrant tropical woody plant, Eurycoma longifolia. Romanian Biotechnological Letters. 17(1): 7026-7025.

    Huỳnh Thị Luỹ, Nguyễn Hữu Hổ & Bùi Văn Lệ (2021). Tạo rễ bất định trực tiếp từ mô lá cây ngũ gia bì chân chim (Schefflera octophylla (Lour). Harms) nuôi cấy in vitro. Tạp chí Công nghệ sinh học. 19(3): 495-507.

    Khalafalla M.M., Daffalla H.M., El-Shemy H.A. & Abdellatef E. (2009). Establishment of in vitro fast-growing normal root culture of Vernonia amygdalina- a potent African medicinal plant. Journal of Central European Agriculture. 8(21): 5952-5957.

    Khanam M.N., Anis M., Javed S.B., Mottaghipisheh J. & Csupor D. (2022). Adventitious root culture an alternative strategy for secondary metabolite production: A review. Agronomy. 12: 1178.

    Khattar S., Khan S.A., Zaidi S.A.A., Darvishikolour M., Farooq U., Naseef P.P., Kurunian M.S., Khan M.Z., Shamim A., Khan M.M.U., Iqbal Z. & Mirza M.A. (2022). Resveratrol from dietary supplement to a drug candidate: An assessment of potential. Pharmaceuticals.15(8): 957.

    Kim J.S., Lee S.Y. & Park S.U. (2008). Resveratrol production in hairy root culture of peanut, Arachis hypogaea L. transformed with different Agrobacterium rhizogenes strains. African Journal of Biotechnology. 7(20): 3788-3790.

    Ling A., Chin M.F. & Hussein S. (2009). Adventitious root production of Centella asiatica in response to plant growth regulators and sucrose concentrations. Medicinal and Aromatic Plant Science and Biotechnology. 3(1): 36-41.

    Ling A.P.K, Tan K.P. & Hussein S. (2013). Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata. Journal of Zhejiang University SCIENCE B.

    : 621-631.

    Medina-Bolivar F., Condori J., Rimando A.M., Hubstenberger J., Shelton K., O’Keefe S.F., Bennett S. & Dolan M.C. (2007). Production and secretion of resveratrol in hairy root cultures of peanut. Phytochemistry. 68(14): 1992-2003.

    Murashige T. & Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Plant Physiology. 15: 473-497.

    Nandagopal S. & Kumari B.D.R. (2007). Effectiveness of auxin induced in vitro root culture in

    chicory. Journal of Central European Agriculture. 8(1): 73-80.

    Ninh Thị Thảo, Nguyễn Thị Phương Thảo, Nguyễn Thị Thùy Linh, Nguyễn Tuấn Minh, Nguyễn Quỳnh Chi & Trần Thị Anh Đào (2016). Nghiên cứu cảm ứng và nuôi cấy rễ bất định cây Ba kích (Morinda officinalis How). Tạp chí Khoa học Nông nghiệp Việt Nam. 14(6): 921-930.

    Nguyễn Thị Liễu, Nguyễn Trung Thành & Nguyễn Văn Kết (2011). Nghiên cứu khả năng tạo rễ bất định của sâm Ngọc Linh (Panax vietnamensis, Ha et Grushv.) trong nuôi cấy in vitro. Tạp chí Khoa học ĐHQGHN, Khoa học Tự nhiên và Công nghệ. 27: 30-36.

    Nguyễn Thị Ngọc Hương & Võ Thị Bạch Mai (2009). Tìm hiểu sự phát sinh hình thái rễ trong nuôi cấy in vitro cây nhàu (Morinda citrifolia L.). Tạp chí Phát triển Khoa học và Công nghệ. 12(17): 100-105.

    Park Y.-E., Park C.-H., Yeo H.-J., Chung Y.-S. & Park S.-U. (2021). Resveratrol biosynthesis in hairy root cultures of tan and purple seed coat peanuts. Agronomy. 11: 975.

    Pop T.I., Pamfil D. & Bellini C. (2011). Auxin control in the formation of adventitious roots. Notulae Botanicae Horti Agrobotanici Cluj-Napoca.

    (1): 307-316.

    Rahmat E. & Kang Y. (2019). Adventitious root culture for secondary metabolite production in medicinal plants: A Review. Plant Biotechnology Journal.

    : 143-157.

    Saiman M.Z., Mustafa N.R., Schulte A.E., Verpoorte R. & Choi Y.H. (2012). Induction, characterization, and NMR-based metabolic profiling of adventitious root cultures from leaf explants of Gynura procumbens. Plant Cell, Tissue and Organ Culture. 109(3): 465-475.

    Salehi B., Mishra A.P., Nigam M., Sener B., Kilic M., Sharifi-Rad M., Fokou P.V.T., Martins N. & Sharifi-Rad J. (2018). Resveratrol: A double-edged sword in health benefits. Biomedicines. 6(3): 91.

    Sharmaa S.N., Jhaa Z. & Sinha R.K. (2013). Establishment of in vitro adventitious root cultures and analysis of andrographolide in Andrographis paniculata. Natural Product Communications. 8(8): 1045-1047.

    Sivakumar G., Yu K.W. & Paek K. Y. (2005). Production of biomass and ginsenosides from adventitious roots of Panax ginseng in bioreactor cultures. Engineering in Life Sciences. 5(4).

    Son S.H., Choi S.M., Hyung S.J., Yun S.R., Choi M.S., Shin E.M. & Hong Y.P. (1999). Induction and cultures of mountain ginseng adventitious roots and AFLP analysis for identifying mountain ginseng. Biotechnology and Bioprocess Engineering. 4: 119-123.

    Sumaryono Wirdhatul M. & Diah R. (2012). Effect of carbohydrate source on growth and performance of in vitro sago palm (Metroxylon sagu Rottb.) plantlets. HAYATI Journal of Biosciences.

    (2): 88-92.

    Taj F., Khan M.A., Ali H. & Khan R.S. (2019). Improved production of industrially important essential oils through elicitation in the adventitious roots of Artemisia amygdalina. Plants. 8: 430.

    Tchouga A.O, Deblauwe V., Djabou S.A.M., Forgione G., Hanna R. & Niemenak N. (2020). Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern. HortScience. 55(4): 424-428.

    Tian L., Zeng Y., Zheng X. & Liu T. (2019). Detection of peanut oil adulteration mixed with rapeseed oil using gas chromatography and gas chromatography-ion mobility spectrometry. Food Analytical Methods. 12(9).

    Trương Quỳnh Như, Võ Thanh Phúc & Lê Thị Thuỷ Tiên (2015). Khảo sát ảnh hưởng của auxin lên sự hình thành và tăng sinh rễ bất định dừa cạn (Catharanthus roseus (L.) G. Don). Tạp chí Phát triển Khoa học và Công nghệ. 18: 75-86.

    Yang T., Fang L., Nopo-Olazabal C., Condori J., Nopo-Olazabal L., Balmaceda C. & Medina-Bolivar F. (2015). Enhanced production of resveratrol, piceatannol, arachidin-1, and arachidin-3 in hairy root cultures of peanut co-treated with Methyl Jasmonate and Cyclodextrin. Journal of Agricultural and Food Chemistry. 63(15): 3942-3950.