Genetic Transformation of Some Cherry Tomato (Lycopersicon esculentum Mill.) Cultivars with The HbsAg Gene

Date Received: 27-07-2014

Date Accepted: 24-09-2014

Date Published: 06-08-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2014.12.7.

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Issue: Vol. 12 No. 7 (2014)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Nam, N., Ha, T., Hao, L., Duc, L., Tri, P., Loc, P., … Ho, N. (2025). Genetic Transformation of Some Cherry Tomato (Lycopersicon esculentum Mill.) Cultivars with The HbsAg Gene . Vietnam Journal of Agricultural Sciences, 12(7), 1005–1014. https://doi.org/10.31817/tckhnnvn.2014.12.7.

Genetic Transformation of Some Cherry Tomato (Lycopersicon esculentum Mill.) Cultivars with The HbsAg Gene

Nguyen Thi Phuong Nam , Tran Thi Ngoc Ha , Le Hoan Hao , Le Tan Duc , Pham Duc Tri , Phan Tuong Loc , Nguyen Huu Tam , Bui Minh Tri , Nguyen Huu Ho (*)

  • Tác giả liên hệ: [email protected]

    • Keywords

    Agrobacterium tumefaciens, cherry tomato (Lycopersicon esculentum Mill.), cotyledon, genetic transformation, HBsAg gene

    Abstract


    The present paper reported the results on the genetic transformation of two cherry tomato cultivars, TN412 (pureline) and TN TN48 (F1 hybrid) with the HBsAg for long-term objective of producing the plant edible vaccine against Hepatitis B virus.  Shoots regenerated from cotyledons served as materials for transformation experiments. The 7-day old cotyledons of tomato seedlings were infected and co-cultivated for 2 days with the Agrobacterium tumefaciens strain carrying the HBsAg gene [driven by the PDS (phytoene desaturase) and T7 promoters], kanamycin resistance gene nptII and gusA reporter gene. Two days after co-cultivation, the cotyledons were washed with  cefotaxime solution and cultured on the MS medium containing + 0.5 mg/l IAA + 2 mg/l BA + 500 mg/l cefotaxime and 30 - 50 mg/l kanamycin for selection of transformants. Through at least 04 rounds of selection (15 - 20 days/round), various kanamycin resistant (KR) lines were obtained in both cultivars and they were confirmed for the presence and expression of transgenes by the GUS assay, by the PCR and Southern blot and western blot analyses. Several T1 individuals from T0 line were evaluated for presence and expression of the nptII gene by the in vitro KR assay and by the PCR analysis. The presence of the HBsAg gene in the T1 individuals also was carried out by the PCR technique.  

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