Characterization of Plant-growth Gromoting Traits of Endophytic Bacterial Strain Enterobacter sp. RC20 Isolated from Roots of Sesbania sesban L.

Date Received: 01-04-2025

Date Accepted: 09-09-2025

Date Published: 30-09-2025

##submissions.doi##: https://doi.org/10.31817/tckhnnvn.2025.23.9.07

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Thuy, N., Hien, N., Bao, P., Nhi, N., & Giang, N. (2025). Characterization of Plant-growth Gromoting Traits of Endophytic Bacterial Strain Enterobacter sp. RC20 Isolated from Roots of Sesbania sesban L. Vietnam Journal of Agricultural Sciences, 23(9), 1201–1210. https://doi.org/10.31817/tckhnnvn.2025.23.9.07

Characterization of Plant-growth Gromoting Traits of Endophytic Bacterial Strain Enterobacter sp. RC20 Isolated from Roots of Sesbania sesban L.

Nguyen Thi Bich Thuy , Nguyen Thi Hong Hien 1 , Pham Gia Bao 1 , Nguyen Yen Nhi 1 , Nguyen Van Giang (*)

  • Tác giả liên hệ: [email protected]
  • 1 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Sesbania sesban L., plant-growth promoting bacteria, IAA, endophytic bacteria, siderophore

    Abstract


    Endophytic bacteria, which are able to live in the tissue of plants, are of interest to scientists due to various benefits they provide to the host plant, such as supplementing phytohormones and enhanced ability to absorb nutrients. This study aimed to select an endophytic bacterial strain from the roots of the Sesbania sesbani L. that can stimulate plant growth. The bacterial strain RC20 was selected from isolated endophytic bacterial strains based on its ability to synthesize high IAA concentration. Comparison of biochemical characteristics and the 16S rRNA nucleotide sequence with those from the GenBank,the strain RC20 belonged to the genus Enterobacter, named as Enterobacter sp. RC20. Strain Enterobacter sp. RC20 exhibited some plant growth-promoting abilities, such as solubilizing phosphate and potassium, and producing siderophores. In vivo experiment Enterobacter sp. RC20 added into the growing medium increased the growth and development of chilli. The plant height and root length of chilli supplemented with Enterobacter sp. RC20 increased by 27.63% and 46.32% in comparison with the control. The fresh weight and dry weight of the treated chilli plants also showed superior growth compared to the untreated ones with a rate of 24.13% and 39.32%, respectively. These findings suggest Enterobacter sp. RC20 as a potential candidate for future research into beneficial agricultural bioproducts.

    References

    Ahmed M., Hussain M., Dhar M.K. & Kaul S. (2012). Isolation of microbial endophytes from some ethnomedicinal plants of Jammu and Kashmir. J Nat Prod Plant Resource. 2(2): 215-220.

    Ames B.N. (1966). Assay of inorganic phosphate, total phosphate and phosphatases. In Book: Methods in enzymology © Elsevier. pp.115-118.

    Bùi Thanh Đạo, Ngô Thanh Phong & Cao Ngọc Điệp (2021). Phân lập và nhận diện vi khuẩn nội sinh có khả năng cố định đạm, hòa tan lân và tổng hợp IAA trong cây đậu phộng (lạc) (Arachis hypogaea L.) trồng tại 03 huyện miền núi tỉnh Bình Định. Tạp chí Khoa học Đại học Cần Thơ. 57(6): 125-131.

    Bộ Khoa học và Công nghệ (2015). TCVN 10784:2015. Vi sinh vật - xác định khả năng sinh tổng hợp axit 3-indole-axetic (IAA).

    Bộ Khoa học và Công nghệ (2010). TCVN 8551:2010. Phương pháp lấy mẫu, chuẩn bị mẫu cây trồng.

    Burragoni S.G. & Jeon J. (2021). Applications of endophytic microbes in agriculture, biotechnology, medicine, and beyond. Microbiological research. 245: 126691.

    Church D.L. (2016). Biochemical Tests for the Identification of Aerobic Bacteria, Section 3. Aerobic Bacteriology, in the book: Clinical Microbiology Procedures Handbook, Editor: Amy L. Leber. Vol. 1‐3, 4th Edition

    Dwivedi M. (2020). Gluconobacter. In Beneficial Microbes in Agro-Ecology. Elsevier. pp. 521-544. doi.org/10.1016/B978-0-12-823414-3.00025-3

    Eid A.M., Salim S.S., Hassan S.E.-D., Ismail M.A. & Fouda A. (2019). Role of Endophytes in Plant Health and Abiotic Stress Management. In Microbiome in Plant Health and Disease. Springer Singapore. pp. 1190144.

    Fanai A., Bohia B., Lalremruati F., Lalhriatpuii N., Lalrokimi Lalmuanpuii R., Singh P.K. & Zothanpuia (2024). Plant growth promoting bacteria (PGPB)-induced plant adaptations to stresses: an updated review. Peer J. 12: e17882.

    Fu S.-F., Wei J.-Y., Chen H.-W., Liu Y.-Y., Lu H.-Y. & Chou J.-Y. (2015). Indole-3-acetic acid: A widespread physiological code in interactions of fungi with other organisms. Plant Signaling & Behavior. 10(8): e1048052.

    Khalifa A.Y., Alsyeeh A.M., Almalki M.A. & Saleh F.A. (2016). Characterization of the plant growth promoting bacterium, Enterobacter cloacae MSR1, isolated from roots of non-nodulating Medicago sativa. Saudi J Biol Sci. 23(1): 79-86.

    Kumar P., Vashistha H. & Kumar S. (2024). PGPR attributes and molecular identification of non-rhizobial Endophytes for growth enhancement of Sesbania sesban (L.) Merr. J. Indian bot. Soc. 104(3): 123-135.

    Lee K.-E., Adhikari A., Kang S.-M., You Y.-H., Joo G.-J., Kim J.-H., Kim S.-J. & Lee I.-J. (2019). Isolation and characterization of the high silicate and phosphate solubilizing novel strain Enterobacter ludwigii GAK2 that promotes growth in rice plants. Agronomy. 9(3): 144

    Makowski K., Leszczewicz M., Broncel N., Lipińska-Zubrycka L., Głębski A., Komorowski P. & Walkowiak B. (2021). Isolation, Biochemical Characterisation and Identification of Thermotolerant and Cellulolytic Paenibacillus lactis and Bacillus licheniformis. Food Technology and Biotechnology. 59(3): 325-336.

    Majeed A., Abbasi M.K., Hameed S., Imran A. & Rahim N. (2015). Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion. Frontiers in Microbiology. 6.

    Masoomi-Aladizgeh F., Jabbari L., Khayam Nekouei R. & Aalami A. (2016). A simple and rapid system for ADN and RNA isolation from diverse plants using handmade kit. Protocol Exchange.

    Moyes R.B. Reynolds J. & Breakwell D.P. (2009). Differential Staining of Bacteria: Gram Stain. Current Protocols in Microbiology. 15(1).

    Mushtaq S., Shafiq M., Tariq M.R., Sami A., Nawaz-Ul-Rehman M.S., Bhatti M.H.T., Haider M.S., Sadiq S., Abbas M.T. & Hussain M. (2023). Interaction between bacterial endophytes and host plants. Frontiers in plant science. 13: 1092105.

    Nguyễn Văn Giang, Vũ Thị Tươi, Vũ Thị Linh & Phạm Hồng Hiển (2021). Ảnh hưởng của môi trường và điều kiện nuôi cấy đến khả năng tổng hợp IAA của một số chủng vi khuẩn nội sinh phân lập từ rễ cây sú (Aegiceras corniculatum). Tạp chí Khoa học và Công nghệ Nông nghiệp Việt Nam. 06(127).

    Nigussie Z. (2013). Sesbania sesban (L.) Merrill: Potential uses of an underutilized multipurpose tree in Ethiopia. African Journal of Plant Science. 7(10): 468-475.

    Ning Z., Lin K., Gao M., Han X., Guan Q., Ji X., Yu S. & Lu L. (2024). Mitigation of Salt Stress in Rice by the Halotolerant Plant Growth-Promoting Bacterium Enterobacter asburiae D2. Journal of Xenobiotics. 14(1): 333-349.

    Nohwar N., Khandare R. & Desai N. (2019). Isolation and characterization of salinity tolerant nitrogen fixing bacteria from Sesbania sesban (L) root nodules. Biocatalysis and Agricultural Biotechnology. 21: 101325.

    Patel S., Jinal H.N. & Amaresan N. (2017). Isolation and characterization of drought resistance bacteria for plant growth promoting properties and their effect on chilli (Capsicum annuum) seedling under salt stress. Biocatalysis and Agricultural Biotechnology. 12: 85-89.

    Phạm Hồng Hiển, Trần Thị Đào, Tạ Hà Trang, Hoàng Thị Liễu & Nguyễn Văn Giang (2022). Ảnh hưởng của môi trường nuôi cấy đến khả năng tổng hợp IAA của chủng vi khuẩn nội sinh HY9 và TT3. Tạp chí Khoa học Nông nghiệp Việt Nam. 20(12): 1640-1648.

    Rout G.R. & Sahoo S. (2015). Role of Iron in Plant Growth and Metabolism. Review in Agricultural Science. 3: 1-24. https://doi.org/10.7831/ras.3.1

    Schwyn B. & Neilands J. (1987). Siderophores from agronomically important species of the Rhizobiacae. Acta Biochim Pol. 41(1): 7-11.

    Setiawati T.C. & Mutmainnah L. (2016). Solubilization of potassium containing mineral by microorganisms from sugarcane rhizosphere. Agriculture and Agricultural Science Procedia. 9: 108-117.

    Wang B., Tan S., Wu M., Feng Y., Yan W., Yun Q., Ji X., Lin R. & Zhao Z. (2024). Effects of two Bacillus velezensis strains isolated from different sources on the growth of Capsicum annum. Frontiers in Microbiology. 15: 1504660.

    Wei H., He W., Li Z., Ge L., Zhang J. & Liu T. (2022). Salt-tolerant endophytic bacterium Enterobacter ludwigii B30 enhance bermudagrass growth under salt stress by modulating plant physiology and changing rhizosphere and root bacterial community. Frontiers in plant science. 13: 959427.