Pathological and Immunohistochemical Characterization of Testicular Tumor (Leydig Cell) in Dogs in Hanoi

Date Received: 21-07-2017

Date Accepted: 02-07-2018

##submissions.doi##:

Views

14

Downloads

4

Issue: Vol. 16 No. 4 (2018)

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Son, N., Nam, N., Lan, N., Dao, B., Nga, B., & Giang, N. (2024). Pathological and Immunohistochemical Characterization of Testicular Tumor (Leydig Cell) in Dogs in Hanoi. Vietnam Journal of Agricultural Sciences, 16(4), 323–331. http://vie.vjas.vn/index.php/vjasvn/article/view/445

Pathological and Immunohistochemical Characterization of Testicular Tumor (Leydig Cell) in Dogs in Hanoi

Nguyen Vu Son 1 , Nguyen Huu Nam (*) 1 , Nguyen Thi Lan 1 , Bui Tran Anh Dao 1 , Bui Thi To Nga 1 , Nguyen Thi Huong Giang 1

  • Tác giả liên hệ: [email protected]
  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Dog, immunohistochemistry, Inhibin-, Leydig (Interstitial) cell tumor, NSE, pathology

    Abstract


    Leydig cell tumor is one of the most common testicular tumors in dogs. The objective of the present study was to examine the clinicopathologic and immunohistochemical features of canine Leydig cell tumor. Testicular tumors (Leydig cells) were found in the testicles of six dogs in veterinary clinics in Hanoi. Clinical symptoms included swelling, hyperpigmentation on the scrotal skin and/or hematuria, perineal hernia, and squatting to urinate. The tumors were macroscopically small, yellow to brown, soft and sharply demarcated with hemorrhage or cysts on cross-section. Microscopically, all cases show a similar histological appearance, being composed of round to polygonal cells with (or without) fine vacuoles in pale, abundant eosinophilic cytoplasm, a small and round eccentric nucleus, and infrequent mitotic figures. Neoplastic cells, in solid sheets (100%), angiomatoid (66.67%) or pseudo-adenomatous (50%) pattern, frequently contained testicular degeneration, lipid accumulation, hemorrhage and cystic structure. Immunohistochemically, tumor cells in all cases were positive for vimentin, inhibin-, melan-A and E-cadherin, but negative for neuron-specific enolase (NSE). These results help in the education and diagnosis of canine Leydig cell tumor and support future studies of tumor in animals.

    References

    Canadas, A., Romão, P. and Gartner, F. (2016) Multiple Cutaneous Metastasis of a Malignant Leydig Cell Tumor in a Dog. J. Comp. Pathology, 155: 181-184.

    Fan, TM. andDe Lorimier, LP. (2007) Tumors of the male reproductive system. Withrow and MacEwen’s Small Animal Clinical Oncology, 4thedition, pp. 637-648.

    Ferrara, N. (2002) Role of vascular endothelial growth factor in physiologic and pathologic angionenesis: therapeutic implications. Semin. Oncol.,29(6 supplement 16): 10-14.

    Grieco, V., Riccardi, E., Greppi, GF.,Teruzzi, F., Lermano, V. and Finazzi, M. (2008). Canine testicular tumours: a study on 232 dogs. J. Comp. Pathology, 138: 86-89.

    Hồ Văn Nam (2001). Chẩn đoán lâm sàng thú y. Nhà xuất bản Nông nghiệp Hà Nội, tr. 20-24.

    Kennedy, PC.,Cullen, JM., Edwards, JF., Goldschmidt, MH., Larsen, S., Munson, L. and Nielsen, S. (1998). Histological classifications of tumors of the genital system of domestic animals. World Health Organization International Histological Classification of Tumors of Domestic Animals, Vol. IV, Armed Forces Institute of Pathology, Washington D.C., pp. 15-16.

    Kimberly, MN.,Erin, MB. andDonna, FK. (2017) Neoplasia and Tumor Biology. In:James F. Z. (Ed.) Pathologic basis of veterinary diseases. 6thed. St. Louis, Missouri: Elsevier, pp. 286-321.

    Nguyễn Thị Lan, Nguyễn Thị Huyên (2012) Nghiên cứu một số đặc điểm bệnh lý của chó bị mắc khối u Lympho và ứng dụng kỹ thuật hoá mô miễn dịch để chẩn đoán bệnh, Tạp chí khoa học kỹ thuật thú y, XIX(4):30-37.

    Nguyễn Vũ Sơn, Nguyễn Hữu Nam, Bùi Trần Anh Đào, Nguyễn Thị Lan, Bùi Thị Tố Nga, Đào Duy Tùng, Nguyễn Thị Hương Giang (2017) Nghiên cứu đặc điểm bệnh lý một số loại u tuyến vú thường gặp trên chó tại Hà Nội. Tạp chí Khoa học kỹ thuật thú y,XXIV(8):68-76.

    Owston, M. and Ramos-Vara J. (2007) Histologic and immunohistochemical characterization of a testicular mixed germ cell sex cord-stromal tumor and a leydig cell tumor in a dog. Veterinary Pathology, 44: 936-943.

    Peters, MA.,de Rooij, DG., Teerds, KJ., van der Gaagand, I. and van Sluijs, FJ. (2000). Spermatogenesis and testicular tumors in ageing dogs. Journal of Reproduction and Fertility, 120: 443-452.

    Peters, MA.,Teerds, KJ., Van der Gaag, I., de Rooij, DG. andvan Sluijs, FJ. (2001). Use of antibodies against LH receptor, 3β‐hydroxysteroid dehydrogenase and vimentin to characterize different types of testicular tumour in dogs. Reproduction, 121: 287-296.

    Rafal Ciaputa, Marcin Nowak, Janusz A. Madej, Dominik Poradowski, Izabela Junus, Piotr Dziegiel, Elzbieta Gorzynska, Malgorzata Kandefer-Gola (2014). Inhibin-a, E-cadherin, calretinin and Ki-67 antigen in the immunohistochemical evaluation of canine and human testicular neoplasms. Folia histochemica etcytobiologica, 52(4): 326-334.

    Ramos‐Vara, JA.,Beissenherz, ME., Miller, MA., Johnson, GC., Kreeger, JM., Pace, LW., Turk, JR., Turnquist, SE., Watson, GL. and Yamini, B. (2001). Immunoreactivity of A103, An antibody to Melan A, in canine steroid‐producing tissues and their tumors. J. Vet. Diagn. Invest.,13: 328-332.

    Taniyama, H., Hirayama, K., Nakada, K., Numagami, K., Yaosaka, N., Kagawa, Y., Izumisawa, Y., Nakade, T., Tanaka, Y., Watanabe, G. and Taya, K. (2001). Immunohistochemical detection of inhibin-alpha, -betaB, and -betaA chains and 3beta-ydroxysteroid dehydrogenase in canine testicular tumors and normal testes. Veterinary Pathology, 38: 661-666.

    Togni, A., Rutten, M., Bley, CR. and Hurter, K. (2015). Metastasized Leydig cell tumor in a dog. Schweizer Archive fur Tierheilkunde, 157: 111-115.