Date Received: 06-02-2025
Date Published: 20-02-2025
##submissions.doi##: https://doi.org/10.1234/m2tp5937
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Characterizing Polyphenol-producing and Plant-growth-promoting Abilities of Endophytic Bacteria Isolated from Stinging Nettle, Urtica dioica L.
,
Do Danh Quang
,
Dinh Mai Van
Keywords
Urtica dioica L., endophytic bacteria, polyphenol, medicinal plants
Abstract
Stinging nettle (Urtica dioica L.) is a medicinal plant widely used in medicine due to its content of diverse biologically active compounds. The objective of this study was to isolate endophytic bacteria (VKNS) from U. dioica L. and identify their biologically active compounds. The plant samples were surface-sterilized and placed on the nutrient agar plates. The results revealed that seven VKNS strains (LG1 to LG7) were obtained from U. dioica L, of which two strains, Bacillus cereus LG1 and Bacillus cereus LG7, had the highest ability to synthesize polyphenols (187.81 and 281.03mg GAE/l, respectively). Moreover, the research revealed that these two strains had other capabilities such as producing extracellular enzymes (amylase, cellulase, and protease), and synthesizing IAA. The supernatant of these two bacteria was resistant to most tested microbial strains and most effective at a concentration of 20 mg/ml. Furthermore, the bacterial supernatant (20 mg/ml) showed the ability to stimulate the germination of the tested seeds. Therefore, the research suggests that B. cereus LG1 and B. cereus LG7 isolated from U. dioica L. were potentially valuable sources of polyphenols. Nevertheless, further research is required to comprehend the process mechanism and achieve effective polyphenol production by endophytic bacteria.
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