ISOLATION AND CHARACTERIZATION OF THE PHYTASE GENE PROMOTER FROM Bacillus licheniformis DSM13

Ngày nhận bài: 20-09-2017

Ngày duyệt đăng: 25-03-2017

Ngày xuất bản: 06-08-2025

DOI: https://doi.org/10.31817/tckhnnvn.2017.15.3.

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Số: Tập 15 Số 3 (2017)

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KỸ THUẬT VÀ CÔNG NGHỆ

Cách trích dẫn:

Trung, N., Trung, N., Thuan, N., Trung, T., Trung, N., Thuy, T., & Anh, N. (2025). ISOLATION AND CHARACTERIZATION OF THE PHYTASE GENE PROMOTER FROM Bacillus licheniformis DSM13. Tạp Chí Khoa học Nông nghiệp Việt Nam, 15(3), 298–305. https://doi.org/10.31817/tckhnnvn.2017.15.3.

ISOLATION AND CHARACTERIZATION OF THE PHYTASE GENE PROMOTER FROM Bacillus licheniformis DSM13

Nguyen Thanh Trung (*) 1, 2, 3, 4 , Nguyen Minh Trung 1, 2, 3, 4 , Nguyen Huy Thuan 1, 2, 3, 4 , Trinh Thanh Trung 1, 2, 3, 4 , Nguyen Quoc Trung 1, 2, 3, 4 , Trinh Thi Thu Thuy 1, 2, 3, 4 , Nguyen Hoang Anh 1, 2, 3, 4

  • Tác giả liên hệ: [email protected]
  • 1 Center for Molecular Biology, Institute of Research and Development, Duy Tan University, Danang
  • 2 Institute of Microbiology and Biotechnology, Vietnam National University, Hanoi
  • 3 Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi
  • 4 Faculty of Food Science and Technology, Vietnam National University of Agriculture, Hanoi

    • Từ khóa

    Bacillus licheniformis, gene expression, phosphate starvation, phytate, phytase

    Tóm tắt


    Phytases are a group of enzymes that hydrolyze phytate to release inorganic phosphate and myo-inositol phosphate intermediates. The phytase encoding gene (phyL) of Bacillus licheniformis DSM13 is strongly induced during phosphate starvation conditions. In this study, we analyzed the activity of the phytase promoter by constructing a recombinant strain containing a plasmid bearing the phyL promoter fragment and the reporter gene xynA (encoding the xylanase) fusion. The recombinant strain was grown in conditions without and with the addition of different concentrations of sodium phytate. The results showed that the phytase promoter was strongly induced when less than 5 mM of phytate was added to the growth medium. Furthermore, growth analysis experiments revealed that phytate was an important alternative phosphate source for B. licheniformis cells to overcome phosphate starvation conditions.

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