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• Single Nucleotide Polymorphisms (SNPs) inthe Gene Segment Coding for Cathepsin DAspartic Proteaseof Zoonotic Hookworm Ancylostoma ceylanicum

  Duong Duc Hieu, Bui Khanh Linh, Nguyen Thu Huong, Le Duc Vinh, Nguyen Thi Hoang Yen, Nguyen Thi Hong Chien, Nguyen Van Phuong

  Date Received: 02-04-2019 / Date Accepted: 01-06-2019

   Abstract PDF (VIETNAMESE)

  This study was conductedto determine the presence of the canine zoonotic hookworm species, Ancylostoma ceylanicum, in humans in Vietnam and to analyze the single nucleotide polymorphism in thegenesegment encoding A. ceylanicumcathepsin D aspartic protease, one of the hydrolytic enzymes with an important role in thelife cycle of hookworm. The existence of Ancylostoma ceylanicumin dogs and humans in Vietnam was confirmedby KOD-PCR. Comparative analysis of tthesequences encoding A. ceylanicumcathepsin D aspartic proteaserevealed, some mutations in exon 7, 8,and 9. Three SNPs non-synonymous mutations (one from exon 7 and two from exon 9)caused the changes in amino acid sequences of aspatyl protease.Our results can be considered forfuther studies on the development of effective vaccine against hookworm diseaseusing recombinant cathepsin D aspartic protease as an antigen.