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• IMMUNO-RELATED GENE EXPRESSION OF ANTI-LIPOPOLYSACCHARIDE FACTORS (ALF) AFTER USING IMMUNOSTIMULANT IN KURUMA SHRIMP (Marsupenaeus japonicus)

  Vu Duc Hanh, Lai Thi Lan Huong

  Ngày nhận bài: 31-07-2025 / Ngày xuất bản: 06-08-2025

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  Supplementation of immuno-stimulant can act as a potent immune modulator and have the effect on inducion of immune- related genes which involved in host defense. In order to clarify the anti-lipopolysaccharide factors (ALF) expression of Kuruma shrimp (Marsupenaeus japonicus) after feeding immuno-stimulant, the supplement containing microalgae and Euglena (Ex400) was selected for the trials. Total RNA was extracted by RNA Iso Plus from the lymphoid organ, intestine and blood of shrimp that was fed with Ex400 diet. Tissues were collected at 0, 3, 7, and 10 days after feeding. ALF transcripts were significantly increased by Ex400 compared to control-diet-fed shrimp (with P<0.01) at 3 days post-feeding in the intestine and lymphoid organ. This suggested Ex test diet might stimulate ALF of Kuruma shrimp immune defence in the intestine and lymphoid organ.

  DOI: https://doi.org/10.31817/tckhnnvn.2017.15.9.