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• Isolation and Selection of Cellulose Degrading Bacteria and Prelimimary Application in Banana by-Product Treatment

  Vu Huynh Huong, Nguyen Vinh Hoang, Nguyen Thi Huyền

  Date Received: 26-12-2023 / Date Accepted: 24-02-2025 / Date Published: 28-02-2025

   Abstract PDF (VIETNAMESE)

  In Vietnam, bananas occupy 19% of the total fruit cultivation area, yielding around 1.4 million tons but only 12% of the volume are utilized, the remaining 88% constitute organic waste. This research aimed to identify and select bacteria capable of breaking down cellulose to treating banana by-products. The Bacillus megaterium H3 strain from 12 soil and banana waste samples was isolated. Optimal conditions were determined for cultivating this bacterium to produce enzymes efficiently. The high-potency cellulase-producing Bacillus megaterium H3 bacteria was found to thrive in a medium with a CMC carbon source and NH4Cl nitrogen source. The most suitable culture conditions for this bacterium were at a temperature of 30°C, over a period of 16 hours, with a shaking speed of 200rpm, and an initial pH of 7. Additionally, supplementing banana stem waste with B. megaterium H3 facilitated 32.28% more efficient cellulose decomposition compared to the control sample. These findings serve as basis for the development of organic fertilizer.

  DOI: https://doi.org/10.31817/tckhnnvn.2025.23.2.11