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• Protocol for Indentification in Tra Catfish (Pangasianodon hypophthalmus) by Morphological Taxonomyand Molecular Genetics methods

  Tran Thi Thuy Ha, Tran Nguyen Ai Hang, Nguyen Thi Huong, Nguyen Thi Thu Thuy

  Date Received: 12-03-2015 / Date Accepted: 30-08-2017 / Date Published: 03-07-2025

   Abstract PDF (VIETNAMESE)

  The combination of morphological taxonomy and molecular genetics methods is required for effective and accurate identification of Tra catfish (Pangasianodon hypophthalmus). The identification based on morphological characteristics is possible to identify accuratelyPangasiidae species that simply relieson number of spines, pelvic fins, pectoral fins, etc. In addition, sequence analysis of cytochrome c oxidase I (COI) andcytochrome b (Cytb) genes along with specialized softwares provide significantly reliable results, which contribute information to confirm the morphological classification. In this study, idetification based on molecular genetics method revealed 99-100% similarity in nucleotide sequences of COI and Cytb genes between 30 fish samples collected in An Giang and Ben Tre provinces with Tra catfish Pangasianodon hypophthalmus species. Our finding confirms the superiority and necessity of combining two identifications methods, morphological taxonomy and molecular genetics methods.

  DOI: https://doi.org/10.31817/tckhnnvn.2017.15.7.