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• The Prevalence of Porcine Epidemic Diarrhea (PED) and Detection of Antibody against PED Virus in Pigs Infected by “Gut Feedback” Method in Thanh Hoa Province

  Hoang Van Son, Pham Ngoc Thach, Nguyen Thi Lan

  Date Received: 10-01-2020 / Date Accepted: 10-07-2020

   Abstract PDF (VIETNAMESE)

  A survey on the prevalence of PED and detection of antibody against PED virus in pigs infected by “gut feedback” method was conducted in Thanh Hoa province. A total of16.443 pigs raised in six districts of Thanh Hoa province was daily observed, the pigs with symptoms of PED were confirmed by PED Ag Test kits and RT-PCR technique.A total of sixty gestation sows (at 13th weeks of pregnancy) was used in an experiment to detect antibodies against PED virus by protein-based enzyme-linked immunosorbent assay (ELISA) testing method. The results showed that the prevalence of PED in pigs in Thanh Hoa province was 14.56% with the case fatality rate (CFR)was 53.38%. Of all districts, the prevalence was the highest in Tinh Gia district (16.12%), while it was the lowest in Yen Dinh district (12.08%). The prevalence and CFR of PED were the highest in suckling pigs (22.01% and 72.63%, respectively) but they were lowest in boars (5.81% and 0%, respectively). In this study, the prevalence of PED was higher in the winter than in the summer (33% and 18%, respectively). The size of farms has also affected the results, in which the large farm showed the highest prevalence (15.79%) but the lowest CFR (50.75%). The results indicated that after infected with PED by “gut feedback” method, all of the serum samples from experimental pigs presented antibodies against PED virus. At 14 days and 21 days after infection, the percentages of pigs showed positive results to antibodies against PED virus were 78.33% and80.00%, respectively.

• Roles of Escherichia coliand Salmonellaspp. in Diarrhoea in Pre- and Post-Weaning Piglets: A Research in An Industrial Farm Model

  Nguyen Anh Tuan, Nguyen Ba Tiep

  Date Received: 17-04-2013 / Date Accepted: 19-06-2013 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  The contribution of Eschrichia coli (E.coli) and Salmonella spp. to diarrhoea of pre- and post-weaning piglets was investigated in Son Tra industrial farm in Bac Ninh province. The isolation rates for E.coli from fecal samples of pre- and post-weaning diarrheic piglets were 86.2 and 78%, respectively. Serotype O149 comprised the highest percentage (48%) followed by O101 (20%); O64 (12%) and O8 (8%) in isolates from pre-weaning piglets. Among isolates from post-weaning piglets, O141 comprised 43.7%, followed by O139 (28.1%), O149 and O138 (9.3% for each serotype). Genes encoding for STa, STb, LT, F4, and F18 were detected at 32; 44; 24; 44 and 32% in isolates from pre-weaning whereas, from fecal samples of post-weaning piglets, the detection rates was 65.5; 21.8; 59.4; 0 and 34.3%, respectively. The virulence was found higher for isolates from pre-weaning piglets. Salmonella spp. were detected only from fecal samples of post-weaning piglets with lower incidence in comparasion to previous reports and all of the isolates were S.typhimurium with very high virulence. High susceptibility of both E.coli and Salmonella isolates to Apramycin, Cephalothin, Amikacin, Certiofur was observed. Taken together, E.coli seemed to play a dominant role in diarrhoea of pre-weaning piglets in an industrial farm and that can be shared by Salmonella spp. in post-weaning counterparts.

  DOI: https://doi.org/10.31817/tckhnnvn.2013.11.3.