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• Ovary Slice Culture Technique for Embyro Rescue of Interspecific Crosses between Lilium longiflorum and L. brownii var. colchesteri

  Nguyen Thi Lam Hai, Hiroshi Okubo

  Date Received: 06-06-2025 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  Lilium brownii var. colchesteri (BRO) has an unique characteristic, i.e., the flower color changes from yellowish-cream to white during anthesis. For introducing this trait to cut flower production in Lilium, interspecific crosses of L. longiflorum (as seed parents) and BRO (as a pollen parent) were carried out. Capsules with unviable seeds were found in all crosses. To overcome the incongruity barrier, ovary slices excised from developing ovaries at 7 to 28 days after pollination (DAP) were cultured in a Murashige and Skoog medium supplemented with 40g·L-1 sucrose, 40g·L-1 D-mannitol and 2.5g·L-1 gellan gum under 24h illumination. The hybridity of germinated bulblets were confirmed before acclimatization by isozyme analysis. Hybrid plantlets were obtained from the crosses of L. longiflorum ‘LPI’ × BRO. After acclimatization and cultivation, 11 hybrid plants produced flowers changing from yellowish cream to white during anthesis as does the pollen parent. 

  DOI: https://doi.org/10.31817/tckhnnvn.2013.11.6.