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• Micropropagation of Four Economically Valuable Cymbidium Species

  Tran Thi Ngoc Lan, Nguyen Hong Hoang, Nguyen Du Sanh, Duong Tan Nhut

  Date Received: 07-08-2014 / Date Accepted: 13-10-2014 / Date Published: 06-08-2025

   Abstract PDF (VIETNAMESE)

  Protocorm-like bodies (PLBs) were formed from apical meristem culture of four Cymbidium species on MS medium supplemented with 0.5 mg/l αNAA., 1 mg/l BA after 45 days under light condition. PLB longitudinal thin cell layer culture was used as a technique for PLBs micropropagation on MS medium containing 0.5 mg/l αNAA, 1 mg/l BA, 10% coconut water (v/v), 30 g/l sucrose, 0.2 g/l glutamine, 1 g/l activated charcoal and 8 g/l agar. These collected PLBs were maintained and proliferated on the same medium after 2 months and then transferred to MS medium supplemented with 0.5 mg/l αNAA, 1 g/l activated charcoal, 30 g/l sucrose, 10% coconut water (v/v) and 8 g/l agar for plantlet regeneration. Histological observation proved that these PLBs originated from somatic embryos. Plantlets regenerated on medium without plant growth regulators after 4 months resulted in well-developed shoots and roots. This investigation is important for micropropagation and preservation of the high-economic Cymbidiums.

  DOI: https://doi.org/10.31817/tckhnnvn.2014.12.7.

• Studies on Agrobacterium tumefaciens - Mediated Transfer of RNAito DendrobiumSonia for Resistance to CyMV

  Nguyen Xuan Dung, Duong Hoa Xo, Nguyen Thi Thanh Thao, Chu Hoang Ha

  Date Received: 30-07-2014 / Date Accepted: 13-03-2015

   Abstract PDF (VIETNAMESE)

  In order to produce a Dendrobiumlinesresistant toCyMV (Cymbidium mosaic virus), protocorm like bodies (PLBs) of DendrobiumSoniawere infected with Agrobacterium tumefaciensC58 carrying plant transgenic vector pK7GWIWG2 (II)/CP, containing RNAi structure of coat protein gene (CP) alone or in combination with ORF2-4 gene of CyMV. The transformed PLBs were disinfected with cefotaxime 300mg/l and screened for kanamycin 500mg/l. Viable PLB-derivedlines were obtained after the screening. The presence of the target gene in these lines was confirmed by PCR.