Date Received: 29-01-2021 / Date Accepted: 07-04-2021
The Bacillusspecies have become an increasingly important group of microorganisms with many applications such as environmental pollution treatment, traditional fermented products, pharmacy, etc. This study collected 66 strains of Bacillusfrom the wastewater samples of rice vermicelli production families at Phu Do to build up a collection of strains of this genus. The activities of extracellular enzymes of 66 isolates were investigated. The results showed that 26/66 strains secreted all three extracellular enzymes as protease, amylase, and cellulase with the largest substrate resolution ring diameters of 18.3, 20.8 and 20.9mm, respectively. Among them, four different strains NTB2.1, NTB2.3, NTB2.11, NTB5.7 were more active in biofilm formation than others based on the crystalline purple dye method. The data from measurement of absorbability at 570nm (OD570) wavelength showed greater than or equal to 3.17. In addition, these strains inhibit the growth of pathogenic microorganisms such as E. coli, Salmolellaand these strains were not antagonized to each other.