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• Evaluating Transferability of Late Blight Resistance from Wild Potato to Cultivated Potato via Protoplast Fusion

  Hoang Thi Giang, Vu Thi Hang, Nguyen Thi Thủy, Do Thi Thu Ha, Ramona Thieme, Thilo Hammann, Nguyen Quang Thach

  Date Received: 30-07-2014 / Date Accepted: 21-10-2014 / Date Published: 06-08-2025

   Abstract PDF (VIETNAMESE)

  This paper presented the evaluation of late blight resistance of somatic hybrid potato plants generated via protoplast fusion between Solanum tuberosum varieties (Delikat, Rasant, Agave, Atlantic) with wild potato species (S. bulbocastanum, S. pinatisectum, S. Tarnii). Late blight resistance was evaluated using detached-leaf method, tuber slice test and field test. The results revealed that only somatic hybrid plants between S. bulbocastanum with cultivated potato variety Delikat showed resistance against late blight disease. Molecular marker analysis for Rpi-blb, resistant-related genes, found the presence of Rpi-blb1 and Rpi-blb3 genes in S. bulbocastanum and its somatic hybrids. This confirmed that late blight resistant genes were successfully transferred from wild potato to cultivated potato via protoplast fusion.

  DOI: https://doi.org/10.31817/tckhnnvn.2014.12.7.