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• Assessment of Genetic Diversity of Sesame Accessions (Sesamum indicumL.) Using SSR and SRAP Markers

  Nguyen Tai Toan, Tran Tu Nga, Vu Van Liet, Nguyen Quoc Trung

  Date Received: 27-06-2016 / Date Accepted: 03-04-2017

   Abstract PDF (VIETNAMESE)

  Sesame (Sesamum indicum L.) is an important oilseed crop with high-quality seed oil and richness inantioxidant substances. The genetic relationship analysis of sesame is inportant for genetic conservation and exploration. In this research, we reportedthe results on analysis of genetic diversity of 56 sesame accessions by using DNA markers. Among 15 markers (10 SSR and 5 SRAP markers)used,3 were monomorphic and 12 were polymorphic with a total of 34 allelesand an average of 2.3 alleles per locus. The proportion of polymorphic bandsranged from 66.7 to 100% and reached 80.9% on average. Genetic similarity coefficient of 56 sesame accessionsranged from 0.52 to 0.97. At 0.70 similarity, 56 accessionswere divided into 7 groups, the largest group with 35 accessions, followed by group 2 with 17 accessions.

• Genetic Diversity and Population Structure of Passion Fruit (Passiflora edulis Sim.) Accessions Revealed by SRAP and ISSR Markers

  Tran Duc Trung, Nguyen Van Viet, Bui Quang Dang

  Date Received: 14-10-2021 / Date Accepted: 01-03-2022

   Abstract PDF (VIETNAMESE)

  Identification of potential germplasms is the crucial step for selection and breeding program of passion fruit (Passiflora edulisSims.). In this study, genetic diversity and population structure of 31 exotic passion fruit accessions (including wild relative P. incarnata accessions) were assessed using sequence-related amplified polymorphism (SRAP) and inter simple sequence repeat (ISSR) markers. By primary screening for marker specificity and polymorphism, 8/15 SRAP markers and 10/18 ISSR were selected for passion fruit genotyping. The heterozygosity index H(0.43), polymorphic information content PIC(0.35), and discriminating power D(0.8) were recorded at a high level implying the significant genetic diversity of the analyzed passion fruit accessions. The Neighbor-Joining phylogenetic tree in combination with population structure model revealed by STRUCTURE discriminated and clustered the studied accessions into five groups which showed high accordance with the fruit color and the origin of the accessions. The analysis of melecular variance (AMOVA) also indicated the high rate of intraspecific and possibility interspecific hybridization among Passifloraspecies. These results provide essential genetic background facilitating efficient conservation and utilization of exotic passion fruit accessions in Vietnam.