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• Optimizaton and Evaluation of Multiplex PCR forAnalysis of Pompano(Trachinotusspp.)

  Luu Thi Ha Giang, Dang Thi Nguyen, Tran Thi Thuy Ha, Phan Thi Van

  Date Received: 04-04-2018 / Date Accepted: 08-05-2018

   Abstract PDF (VIETNAMESE)

  In this study, fifteen microsatellite markers were used to optimize a protocol for analyzing genetic diversityof pompano (Trachinotusspp.). Seven single markers were successfully optimized for short fin Pompano and four markers were optimized for long fin pompano. Three multiplex PCR reactionsincludingtwo multiplex pools for short fin Pompano and one pool for long fin pompano were developed which composed of nine fluorescent-labeled markers. Fragment analysis process was successfully established with three pools of microsatellite markerscorresponding to nine loci. Genotyping results revealedpolymorphism in eight loci (EC-7; EC-9; EC-10; EC-17; EC-20; EC-28; TB018; TBG030) with the number of alleles rangingfrom 3 to 6, whereas locus TO67 showed monomorphisms. Thesefindingsimportantlycontributeto the understanding of genetic diversity in pompano.