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• Isolation and Identification Biochemical Characteristics of Porcine Circovirus Type 2 (PCV2) from pigs in Some Northern Provinces of Vietnam

  Huynh Thi My Le, Nguyen Van Giap

  Date Received: 15-04-2013 / Date Accepted: 15-06-2013 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  The aim of this study was to isolate and identify biochemichal characteristics of PCV2formpigs with PCVAD in 4 nothern provinces of Vietnam (Hanoi, Hoa Binh, Bac Giang and Hai Duong). Isolation of PCV2 would be a conclusive evidence to confirm the circulation of PCV2 and PCVAD in pigs of Vietnam. 8 of PCV2 were isolated from 38 samples collected from pigs with suspected PCVAD. PCV2 isolates were PCV2b genotype. The result of identification biochemical characteristics of isolated PCV2 shown that PCV2 could multiply in PK15 cell culture from the first or second passage, but no CPE was observed. TCID50of all ranging from 102,7to104,2 /ml.

  DOI: https://doi.org/10.31817/tckhnnvn.2013.11.3.

• Biological Characterization of Canine Parvovirus Type 2 IsolatesCausing Canine Parvovirus Enteritis in North Viet Nam

  Truong Quang Lam, Nguyen Thi Lan, Nguyen Anh Tuan, Nguyen Thi Yen, Nguyen Thi Hoa, Le Thi Luyen

  Date Received: 10-03-2021 / Date Accepted: 21-01-2022

   Abstract PDF (VIETNAMESE)

  Canine parvovirus (CPV) is a highly contagious viral disease that commonly causes acute gastrointestinal illness in dog breeds in Vietnam. We isolated and determined genotype and biological characteristics of CPV in the CRFK cell line. Three strains of CPV, VNUA.CPV.HN01, VNUA.CPV.HN04 and VNUA.CPV.HY05 in the CRFK cell line were isolated from 30 clinicalsamples. The phylogenetic analysis revealed that all CPVisolates belonged to the genotype CPV2c. CPV did not cause disease in the first passage but caused cytopathogenic effect in the CRFK cells after 2 to 4 blind passages. Cytopathic effect (CPE) occurred within 24 hours post-infection and the cells were completely destroyed after 60 to 72 hours of infection. CPE viral titer was high, at 107,47TCID50/ml. The growth curve reached the peak at 60 to 72 hours after infection and then decreased gradually.

• Biological Characterization of Canine Parvovirus Type 2 IsolatesCausing Canine Parvovirus Enteritis in North Viet Nam

  Truong Quang Lam, Nguyen Thi Lan, Nguyen Anh Tuan, Nguyen Thi Yen, Nguyen Thi Hoa, Le Thi Luyen

  Date Received: 10-03-2021 / Date Accepted: 21-01-2022

   Abstract PDF (VIETNAMESE)

  Canine parvovirus (CPV) is a highly contagious viral disease that commonly causes acute gastrointestinal illness in dog breeds in Vietnam. We isolated and determined genotype and biological characteristics of CPV in the CRFK cell line. Three strains of CPV, VNUA.CPV.HN01, VNUA.CPV.HN04 and VNUA.CPV.HY05 in the CRFK cell line were isolated from 30 clinicalsamples. The phylogenetic analysis revealed that all CPVisolates belonged to the genotype CPV2c. CPV did not cause disease in the first passage but caused cytopathogenic effect in the CRFK cells after 2 to 4 blind passages. Cytopathic effect (CPE) occurred within 24 hours post-infection and the cells were completely destroyed after 60 to 72 hours of infection. CPE viral titer was high, at 107,47TCID50/ml. The growth curve reached the peak at 60 to 72 hours after infection and then decreased gradually.

• Molecular Characterization of a Duck Adenovirus Type 3 (VNUA-DAdV3-1) Strain Isolated from a Diseased Duck in Bac Ninh province, Vietnam

  Trương Quang Lâm, Nguyễn Anh Tuấn, Đào Lê Anh, Nguyễn Thị Lan

  Date Received: 07-04-2026 / Date Accepted: 02-10-2026 / Date Published: 26-10-2026

   Abstract PDF (VIETNAMESE)

  Duck adenovirus type 3 (DAdV-3-1), a member of the Aviadenovirus, is an important pathogene causing significant economic losses to the duck industry in China. However, studies and information regarding the circulation of DAdV-3 in duck populations in Vietnam remain limited. This study was aimed to isolate and molecularly characterize a DAdV-3 strain, designated VNUA-DAdV3-1, from clinical samples collected from a diseased duck flock in December 2025 in My An, Bac Ninh province, Vietnam. The VNUA-DAdV3-1 strain was successfully isolated and propagated in duck embryo fibroblast (DEF) cells. Cytopathic effects (CPE) were first observed at 24h post-inoculation, became evident at 48 and 72h, and reached maximum severity at 96 h, characterized by cell rounding, shrinkage, detachment, and extensive destruction of the cell monolayer with the viral titer peaked at 1.36 × 10⁷ TCID50/ml. PCR and real-time PCR assays confirmed that the isolate belonged to DAdV-3. Sequence analysis of the Hexon gene revealed more than 99% nucleotide identity with DAdV-3 strains reported in China during 2015-2022. To our knowledge, this study represents the first documentation of DAdV-3 isolation and confirmation of its circulation in Vietnam. These findings provide essential baseline data for epidemiological surveillance and the development of effective control strategies against Duck adenovirus type 3 infection in duck populations.

  DOI: https://doi.org/10.31817/tckhnnvn.2026.24.SP01.12