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• Molecular Characterizations of Porcine Epidemic Diarrhea Virus (PEDV) Isolated in Hung Yen Province

  Nguyen Van Giap, Dang Huu Anh, Truong Ha Thai, Huynh Thi My Le

  Date Received: 19-05-2020 / Date Accepted: 02-06-2020

   Abstract PDF (VIETNAMESE)

  Since the first detection in 2009, PEDV has been circulating in the North, Centre and the South of Vietnam. The disease has caused significant damages to the pig production sector. In order to mitigate the economic damage caused by diseases, the manufacture and use of field- matching vaccines are necessary. Aimed at providing more information on the biology of pathogenic PEDV strains in Vietnam, this study analyzed the molecular biology of the gene encoding S1 domain of one PEDV isolate detected in Hung Yen in 2017. The results showed that the field isolate, PEDV_HY2017, is genogroup G2 PEDV. The virus shared nucleotide similarity with PEDV strains of Vietnam in the ranges of 90-91% and 95.7-98.2% in compared to genogroup G1 and genogroup G2, respectively. The PEDV_HY2017 strain carries two regions of insertion-deletion mutations in the gene segment encoding the S1 domain of the Spike protein. This characteristic was similar to the other Vietnamese PEDV genogroup G2 viruses.

• Molecular Characterization of ThePorcine Epidemic Diarrhea Viruses Isolated in Quang Tri, Thai Nguyen, and Thai Binh During 2013 - 2014

  Le Van Phan, Nguyen Trung Tien, Vu Thi Thu Hang, Huynh Thi My Le, Nguyen Ba Hien

  Date Received: 22-07-2015 / Date Accepted: 12-10-2015 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  Porcine Epidemic Diarrhea (PED) is a serious and contagious swine disease caused by a virus ofCoronaviridaefamily. PED occurs throughout the year but more common in winter season and over 90% of cases occur in less than 7 day old piglets. In this study, 148 samples of faeces and intestines collected from PED-suspected pigs in the 3 provinces of Quang Tri, Thai Nguyen and Thai Binh during 2013 - 2014 werediagnosed by the RT-PCR method. The diagnostic results showed that 57/148 (38.51%) samples were positive with the PED virus. The nucleotide (nt) and deduced amino acid (aa) analyses based on the partial spike (S) gene sequencing showed that the present PED virus strains were closely related to the reference PED virus strains collected in VietNam previously, sharing 94.8 - 98.6% in nt and 93.5 - 98.8% in aa. The phylogenetic analysis revealed that all the present PED virus strains belonged to the group G1-1.

  DOI: https://doi.org/10.31817/tckhnnvn.2015.13.7.