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• Development of Colorimetric Sensor Using Gold Nanoparticle and Thymine-Single Stranded DNA for Rapid and Selective Detection of Mercury Ions in Water

  Dong Huy Gioi, Bui Thi Thu Huong, Phi Thi Cam Mien, Nguyen Thi Thuy Hanh, Do Duc Nam

  Date Received: 21-12-2015 / Date Accepted: 11-03-2016 / Date Published: 12-06-2025

   Abstract PDF (VIETNAMESE)

  In this study, we have developeda colorimetric sensor for rapid and selective detection of mercury ions (Hg2 + ) in water by using the gold nanoparticle solution (AuNPs) and thymine-single stranded DNA (T-ssDNA). AuNPs is used as a sensing element based on their unique surface plasmon resonance propertiesand the T-ssDNA isself-assembled on gold nanoparticles to produce the AuNPs-T-ssDNA complex. Single-stranded DNA (T-ssDNA) could enhance the AuNPs against NaNO3-induced aggregation. However, the presence of mercury ions in the complex of AuNPs-T-ssDNA will reduce the stability of AuNPs due to the formation of Hg2 + mediated T-Hg2 + -T base pairs accompanied with the AuNPs color change from red to purple or even to dark blue. As a result, Hg2 + can be detected qualitatively or quantitatively by the naked eye or by UV-vis spectral measurement. The lowest detectable concentration of mercury ions by naked eye and by the UV-vis spectral measurement was 0.06µMand1nM, respectively.

  DOI: https://doi.org/10.31817/tckhnnvn.2016.14.3.