Date Received: 17-09-2013 / Date Accepted: 26-09-2013 / Date Published: 02-07-2025
In hot and humid climate countries, such as Vietnam, Magnaporthe grisea causes serious damage to rice. MPG1, a pathogenicity gene of Magnaporthe grisea is related to the process of aspersorium formation, symptom development and sporulation. Therefore, inhibition of MPG1 gene expression probably results in reduced or loss of pathogenicity of Magnaporthe grisea. A coding fragment of MPG1 gene of 4 rice blast fungus isolates was cloned and the sequencing results showed that the values of gene identity ranged from 99.3-100% among isolates in comparison with the control strain (Guy-11, ID genbank: L20685.2). Two artificial miRNAs were designed using natural Arabidopsis thaliana miRNA-319a backbone to specifically inhibit MPG1 gene expression. These artificial miRNAs were ligated into plant expression pPS1 vector to create gene construct for transferring into rice plant via Agrobacterium tumefaciens. 18 lines of transgenic rice variety J02 were identified to carry pre-amiR-P1 and these lines showed adaptation and survival under the greenhouse condition.
