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Effects of Polymorphism of PIT1, H-FABP, PIK3C3 and CAST Genes on Carcass Traits and Meat Quality of Vietnamese Native Pig (Pot-bellied Pig)

Phan Thi Tuoi, Tran Xuan Manh, Nguyen Van Hung, Nguyen Thai Anh, Nguyen Hoang Thinh, Do Duc Luc

Date Received: 06-02-2023 / Date Accepted: 27-03-2023
The aims of study were to evaluate the effects of polymorphisms of PIT1/RasI, H-FABP/HinfI, PIK3C3/Hpy8IandCAST/HinfIgenesand gender on carcass traits and meat quality of Vietnamese native pot-bellied pigs raised at DABACO Breeding Pig Company, Phu Tho Province, Vietnam. At 8 months of age, 23pigs (12 gilts and 11 barrows) were slaughtered to measure carcass traits (live body weight, hot carcass weight, killingout percentage, dress carcass weight, carcass yield, carcass length, backfat thickness at the vertebra prominensC7 and the last lumbar vertebra L5) and meat quality (pH value of longissimus dorsimuscleat 45 minutes (pH45) and 24 hours post mortem(pH24), drip loss percentage, cooking loss percentage, L* (lightness), a* (redness) và b* (yellowness), shear force at 24h post mortem). The polymorphisms of PIT1/RasI, H-FABP/HinfI, PIK3C3/Hpy8IandCAST/HinfIgenes were identified by PCR-RFLP technique. The results indicated that backfat thickness and L* of longissimus dorsimusclewere higherthan those in barrows(P<0.05). The polymorphisms of PIT1/RasI, H-FABP/HinfI, PIK3C3/Hpy8IandCAST/HinfIgenes did not affect carcass traits and meat quality of the nativepigs.
Evaluation of 24-bp Insertion-Deletion and C2402T Polymorphisms of the Prolactin Gene in Two Vietnamese Native Chickens: Riand Mia Chicken

Nguyen Hoang Thinh, Nguyen Thi Chau Giang

Date Received: 01-03-2018 / Date Accepted: 20-06-2018
The Vietnamese native chickens have low egg production so they is difficult to breeding andtheir population is declining.Polymorphisms (24-bp Insertion-Deletetion và C2402T) of Prolactin geneis usually considered as candidate genes in the genetic analysis for the improved performance traits in poultry. The study was conducted to determine two SNPs polymorphisms (24-bp Insertion-Deletetion and C2402T) of Prolactin gene in two Vietnamese native breeds Ri and Mia chicken.For genotyping of individual was identified by PCR and PCR-RFLP methods. The results showed that the individual’s genotype was identified in two Riand Mia chicken breed. For 24-bp Insertion-Deletetion polymorphism, the II genotype in Ri chicken and Mia chicken present at low frequencies, (0.07) whereas for DD genotypes in Ri and Mia chickens occur at high frequencies, 0.68 and 0.82, respectively. For C2402T polymorphism, the study shown that two breeds (Ri and Mia) had the highest TT genotypes, respectively 0.64 and 0.84; The CC genotype in both Ri and Mia chickens appeared at low frequencies of 0.08 and 0.07, respectively.
Polymorphisms of Growth Hormone, Growth Hormone Receptor Genes and Their Association with Body Weight of Lien Minh Chicken

Do Thi Thu Huong, Nguyen Thai Anh, Nguyen Thi Vinh, Do Duc Luc, Bui Huu Doan, Nguyen Hoang Thinh

Date Received: 29-03-2023 / Date Accepted: 29-06-2023
This study was carried out to determine the polymorphism of growth hormone (GH), growth hormone receptor (GHR) genes and the association of the polymorphisms with body weight in Lien Minh chicken. Total 835 birds (329 males, 506 females) with body weight form 1 day old to 20 weeks of age were observed. Genomic DNA was extracted form blood and analyzed for single nucleotide polymorphisms (SNPs) by PCR-RFLP. For G662A/GH, three genotypes, AA, AG and GG were found with the frequency of allele A was 0.91 and allele G was 0.09; for G1705A/GH, also 3 genotypes: AA, AG and GG with allele A frequency of 0.18 and allele G of 0.82 were identified; for C571T/GHR polymorphism, 3 genotypes: CC, CT and TT with frequency of allele C was 0.74 and allele T was 0.26. Chickens with the GG genotype of G662A/GH polymorphism had a significantly higher body weight than the individuals with the AA genotypes from 11 to 20 weeks of age (P <0.05). For the G1705A/GH polymorphism, chickens with AA genotype had a significantly higher weight than chickens with AG genotype from 12 to 17 weeks of age (P <0.05), and significantly higher than chickens with GG genotype from 8 to 20 weeks of age (P <0.05). No association between C571T/GHR polymorphism with body weight trait in Lien Minh chickens (P >0.05) was detected in this study.
Single Nucleotide Polymorphisms of Insulin-like Growth Factor Binding Protein 2 in Chicken

Do Vo Anh Khoa, Nguyen Thi Kim Khang, Nguyen Minh Thong, Bui Xuan Men

Date Received: 23-10-2012 / Date Accepted: 28-12-2012
The present study conducted on three different breeds of Tau Vang (n=237), Noi (n=34) and commercial Cobb 500 chickens (n=23) aimed at evaluating single nucleotide polymorphims in IGFBP2 (Insulin-like growth factor binding protein 2) using PCR-RFLP method. Three poplymorphisms at positions g.639G>A (exon 2), g.1023C>T (intron 2) and g.738G>A (exon 3) were detected based on restriction enzymes Bsh1236I, Eco72I and Alw21I, respectively. Genotypic and allelic frequencies at these polymorphisms, which were also identified in previous studies, have effects on economic traits in various chicken populations and follow Hardy-Weinberg equilibrium. These can be considered as important evidences for developing further studies for IGFBP2 polymorphic association with growth, meat yield and meat quality traits in Tau Vang chicken.
Growth Performances of Mia Chicken Carrying Polymorphisms of the INS and GH genes

Hoang Anh Tuan, Nguyen Hoang Thinh, Pham Kim Dang, Bui Huu Doan

Date Received: 29-07-2021 / Date Accepted: 09-12-2021
This study was conducted to evaluate the growth performances of the Mia chicken carrying polymorphisms of the INS and GH genes. A total of 1,100 newly hatched of Mia chickens with characteristic morphological features, were selected from the Mia population in Son Tay town, and raised for evaluation of growth performance at Hadinco Farm. At 8 weeks of age, blood samples were taken from the wing veins of each individual and were identified the genotype by PCR-RFLP method.The results showed that the A3971G polymorphism of the INS gene had 3 genotypes AA, AG and GG with a frequency of 29%, 55% and 16%, respectively. The T3737C polymorphism had 2 genotypes CT (78%) and TT (22%); no CC genotype was found. The C423T polymorphism has 3 genotypes CC, CT and TT with frequencies of 21%, 45% and 34%, respectively. The polymorphism of G662A had 3 genotypes, in which genotypes AA and AG had frequencies of 51% and 40%, respectively. All of the above genotypes did not have a significant effect on the weight of Mia chickens, while those carrying GG genotype of the G662A had bodyweight higher by 10.4% in the general population. However, the number of Mia chickens carrying this genotype in the population was very low, accounting for only 9%.
Single Nucleotide Polymorphisms (SNPs) inthe Gene Segment Coding for Cathepsin DAspartic Proteaseof Zoonotic Hookworm Ancylostoma ceylanicum

Duong Duc Hieu, Bui Khanh Linh, Nguyen Thu Huong, Le Duc Vinh, Nguyen Thi Hoang Yen, Nguyen Thi Hong Chien, Nguyen Van Phuong

Date Received: 02-04-2019 / Date Accepted: 01-06-2019
This study was conductedto determine the presence of the canine zoonotic hookworm species, Ancylostoma ceylanicum, in humans in Vietnam and to analyze the single nucleotide polymorphism in thegenesegment encoding A. ceylanicumcathepsin D aspartic protease, one of the hydrolytic enzymes with an important role in thelife cycle of hookworm. The existence of Ancylostoma ceylanicumin dogs and humans in Vietnam was confirmedby KOD-PCR. Comparative analysis of tthesequences encoding A. ceylanicumcathepsin D aspartic proteaserevealed, some mutations in exon 7, 8,and 9. Three SNPs non-synonymous mutations (one from exon 7 and two from exon 9)caused the changes in amino acid sequences of aspatyl protease.Our results can be considered forfuther studies on the development of effective vaccine against hookworm diseaseusing recombinant cathepsin D aspartic protease as an antigen.
NghiêncứutínhđahìnhExon 14, gen MX ở mộtsốgiốnggàbảnđịacủaViệtNam

Bui Tran Anh Dao, Dong Thi Hong Lien, Nguyen Thi Phuong Thao, Daniel Desmecht

Date Received: 05-05-2013 / Date Accepted: 25-08-2013
Nghiên cứu tính đa hình của exon 14, gen Mx được thực hiện trên 7 giống gà bản địa của Việt Nam (bao gồm: gà Ác, Đông Tảo, H’Mông, Hồ, Mia, Móng và Ri). Kết quả cho thấy tính đa hình của exon 14 xảy ra tại hai vị trí (2032 và 2159). Sự thay thế A hoặc G tại vị trí 2032 của gen Mx có ở tất cả 7 giống gà bản địa được sử dụng trong nghiên cứu này. Trong cùng một giống đều có cả hai alen A và G, tuy nhiên, alen A (liên quan đến tinh kháng virus) có tần số xuất hiện cao hơn so với alen G (liên quan đến tính mẫn cảm với virus). Đặc biệt, tần số của alen A ở giống gà Ri và gà H'mong là cao nhất trong số 7 giống được kiểm tra (với tỉ lệ tương ứng là 80,91% và 74,04%). Phân tích so sánh các axit amin cho thấy sự thay đổi nucleotide A hoặc G tại vị trí 2032 sẽ dẫn đến sự thay đổi axit amin tại vị trí 631 của protein Mx từ Asparagine (liên quan tới khả năng kháng virus của giống) sang Serine (không liên quan tới khả năng kháng virus của giống).
Effect of MyoGand Lif Gene on Economic Traits in Pigs

Do Vo Anh Khoa

Date Received: 14-07-2012 / Date Accepted: 20-08-2012
The study was conducted in 33 castrated male pigs of Yorkshirex Landrace crossbredto analyze the association of polymorphisms of myogenin (MyoG) and Leukemia-Inhibitory-Factor (LIF)genes with some economic traits for growth and carcass performance. The genotypes of MyoG were significantly associated with carcass percent, in which pigs with heterozygous genotype AB (76.32±1.30) was superior to the homozygous genotypesBB (72.77±1.30) and AA (69.46±2.91) (P=0.05). In addition, in LIF locus, the association between genotypes and carcass length also showed significant difference (P<0.05), with the highest carcass length occurring in pigs withhomologous genotype BB (78.00±2.84). Therefore, it is suggested that allele “B” in both candidate genes could be used as a SNP-marker to improve carcass performance in pigs.
Association of Single Nucleotide Polymorphism of the GHSR Gene with Meat Quality Traits in Tau VangChicken

Do Vo Anh Khoa, Chau Thien Ngoc, Le Cong Trieu, Nguyen Huy Tuong

Date Received: 08-08-2016 / Date Accepted: 06-03-2017
Growth hormone secretagogue receptor (GHSR) is one of the candidate genes for meat quality of chicken. The study focused on the association between the genetic polymorphism of the GHSR gene with meat quality traits in Tau Vang chickens. Two polymorphisms at nucleotide G656A and C3678T were identified by PCR/RFLP technique using restriction enzyme MspI and Bsp119I, repectively. The genotypic frequencies at G656A and C3678T loci were found in Hardy-Weinberg equilibrium. The study showed that the G656A polymorphism exerted influence onash content of breast muscleand there wassignificant associations between C3678T polymorphism and dry matterandether extract of leg muscle in Tau Vang chickens.
NghiêncứukhảnăngkếthợpvàsửdụngchỉthịphântửSSR dòtìmgen lg1vàlg2tronglaiđỉnhhaidòngthửMo17 vàB73 vớicácdòngtựphốingôláđứng

Hoang Thi Thuy, Vu Thi Bich Hanh, Tran ThiThanh Ha, Duong Thi Loan, Nguyen Van Ha, Vu Van Liet

Date Received: 07-08-2015 / Date Accepted: 05-05-2016
Nghiên cứu thực hiện đánh giá khả năng kết hợp chung của tám dòng tự phối ngô về tính trạng lá đứng và năng suất hạt sử dụng mô hình line × tester trong vụ xuân 2015; và để phát hiện hai gen lg1 và lg2trong các dòng bố mẹ này cũng như con lai F1sử dụng chỉ thị phân tử SSR. Mười sáu tổ hợp lai đỉnh và các dòng bố mẹđược đánh giá trong vụ xuân 2015 trong thí nghiệm khối ngẫu nhiên hai lần lặp lại. Kết quả xác định góc lá trung bình của ba lá trên bắp nhận thấy dòng E1, E5 và cây thử Mo17 có góc lá từ 30-35othuộc nhóm lá gọn, các dòng còn lại có góc lá < 30othuộc nhóm lá đứng. Giá trị hướng lá (LOV) cũng cho thấy kiểu cây của các dòng và tổ hợp lai thuộc nhóm cây gọn. Chúng tôi xác định chỉ có tổ hợp lai 15 có góc lá <30o thuộc nhóm lá đứng, sáu tổ hợp lai có góc lá >35othuộc nhóm lá thường, và các tổ hợp lai còn lại thuộc nhóm lá gọn. Ước lượng giá trị khả năng kết hợp chung (KNKH) của 8 dòng và 2 cây thử, kết quả cho thấy3 dòng là E4, E7, E8 và cây thử Mo17 có giá trị âm KNKH về góc lá, nghĩa là góc lá có xu hướng hẹp hơn. Sáu dòng có giá trị KNKH dương về năng suất là E1, E2, E3, E4, E6 và Mo17. Sử dụng chỉ thị SSR với hai mồi đặc hiệu umc1165(dò tìm gen lg1) và bnlg1505(dò tìm gen lg2) ở các dòng bố mẹ, THL, kết quả cho thấy mức độ đa hình cao và đã nhận biết được các dòng và THL mang gen lg1và lg2. Kết quả này gợi ý rằng có thể sử dụng chỉ thị phân tử SSR trong chọn lọc trợ giúp nhờ chỉ thị phân tử (MAS) để sàng lọc vật liệu và chọn giống ngô lá đứng cho trồng mật độ cáo.
Methods for Findings Single Nucleotide Polymorphisms

Nguyen Van Dinh, Pham Ha Thuy

Date Received: 05-01-2012 / Date Accepted: 15-05-2012
Single nucleotide polymorphism (SNP), one of the most popular variant of the DNA of the organism. The SNP studies have important roles in biological research, gene mapping, selected animals and plants. The contents of the article presents an overview of SNP detection methods are now widely used around the world.

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