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• Isolation and Serotyping of Haemophilus parasuisStrains from Pigs Raised in the Provinces of Thanh Hoa, Hung Yen,andHa Nam of Northern Vietnam

  Truong Quang Lam, Nguyen Thi Lan, Nguyen Thi Hoa, Nguyen Thi Huyen

  Date Received: 30-07-2018 / Date Accepted: 05-03-2019

   Abstract PDF (VIETNAMESE)

  The aim of this study was to determine the serotypes of Haemophilus parasuisstrains isolated from suspected pig cases of glasser disease. A total of 205 specimens including nasal,trachea, joint fluids, heart and lung collected from 41 suspected pigs inThanh Hoa, Hung Yen and Ha Nam province were used for bacterial isolation and identification. The isolates (22 isolatesin total)ofH. parasuisfrom pigs suspected of being infected withH. parasuisshowedthe major characteristics of small, transparent, and non-hemolytic colonies,gram negative, pleomorphic rod from short to long bacilli,catalase, oxidaseandV factor positive, indole and urease negative, and capable of fermentatingglucose, galactose and fructose, and unable to grow on MacConkey agar. Theseisolates were confirmed by specific PCR asH. parasuis. In the present study, for the first time, we reportedthe application of multiplex PCR assay for serotypedetermination ofH. parasuisisolates from the field in Vietnam.mPCRresults showed variable distributions in the serotypesofH. parasuisisolated from the field. Remarkably,serotypes4, 5 and 1 were the most prevalent with 25%, 25% and15%, respectively, followed by serotypes13 and 2 with 5%, and non-typable serotypewith 20%.

• Characterization of Salmonella Serotypes from Postweaning Pigs with Diarrhea in Some Industrial Farms in Northern Vietnam

  Nguyen Manh Phuong, Nguyen Ba Tiep, Van Thi Huong, Cu Hữu Phú

  Date Received: 12-01-2012 / Date Accepted: 10-04-2012 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  Salmonella isolates from faeces and organs of postweaning pigs with diarrhea in 9 industrial farms in 6 northern provinces of Vietnam comprised 5 serotypes. S. typhimurium serotype made up 51.61% of the isolates, followed by S. anatum (19.35%), S. agona (16.14%), S. meleagridis and S. ruzizi (6.45% for each serotype). All of the isolates were resistant to streptomycin and most of them were resistant to tetracyclin, amoxicillin, ampicillin and sulfatrimethoprime along with very high virulence. The snt gene coding for enterotoxin production and invA gene coding for invasion factor were detected in most of the isolates. These two genes were detected in all of the S. typhimurium isolates. The results indicated that S. typhimurium may play an important role in diarrhea symptom of pigs in the northern provinces of Vietnam.

  DOI: https://doi.org/10.31817/tckhnnvn.2012.10.2.

• Prevalence of serotypes and antibiotic resistance of Salmonella strains isolated from pig slaughterhouses in Hung Yen province

  Lai Thi Lan Huong, Trinh Dinh Thau

  Date Received: 12-07-2017 / Date Accepted: 23-08-2017 / Date Published: 03-07-2025

   Abstract PDF (VIETNAMESE)

  In this study, we determined serotypes and antibiotic resistance of Salmonella isolates from several samples (including water samples, caecal contents, and swab samples) collected from two pig slaughterhouses in Hung Yen province) . 43/48 isolates strains were identified as belong to 9 serovars of Salmonella and 5/48 strains that did not identify serotypes. S.derby was the most common 20,83% and then S. typhimurium (18,75), and S. enteritidis (10,42%). Isolated strains in this study are strongly resistant to Streptomycin 96,67%, Tetracyline 83,33%, Ampicillin 63,33%, Trimethoprim 46,67%, Gentamycin 33,33%, Nalidixic acid 36,67%. Samonella isolated strains are susceptible to some new antibiotics such as Nofloxacin, Nitrofurantion , Ciprofloxacin, Ceftazidime.

  DOI: https://doi.org/10.31817/tckhnnvn.2017.15.7.

• Roles of Escherichia coliand Salmonellaspp. in Diarrhoea in Pre- and Post-Weaning Piglets: A Research in An Industrial Farm Model

  Nguyen Anh Tuan, Nguyen Ba Tiep

  Date Received: 17-04-2013 / Date Accepted: 19-06-2013 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  The contribution of Eschrichia coli (E.coli) and Salmonella spp. to diarrhoea of pre- and post-weaning piglets was investigated in Son Tra industrial farm in Bac Ninh province. The isolation rates for E.coli from fecal samples of pre- and post-weaning diarrheic piglets were 86.2 and 78%, respectively. Serotype O149 comprised the highest percentage (48%) followed by O101 (20%); O64 (12%) and O8 (8%) in isolates from pre-weaning piglets. Among isolates from post-weaning piglets, O141 comprised 43.7%, followed by O139 (28.1%), O149 and O138 (9.3% for each serotype). Genes encoding for STa, STb, LT, F4, and F18 were detected at 32; 44; 24; 44 and 32% in isolates from pre-weaning whereas, from fecal samples of post-weaning piglets, the detection rates was 65.5; 21.8; 59.4; 0 and 34.3%, respectively. The virulence was found higher for isolates from pre-weaning piglets. Salmonella spp. were detected only from fecal samples of post-weaning piglets with lower incidence in comparasion to previous reports and all of the isolates were S.typhimurium with very high virulence. High susceptibility of both E.coli and Salmonella isolates to Apramycin, Cephalothin, Amikacin, Certiofur was observed. Taken together, E.coli seemed to play a dominant role in diarrhoea of pre-weaning piglets in an industrial farm and that can be shared by Salmonella spp. in post-weaning counterparts.

  DOI: https://doi.org/10.31817/tckhnnvn.2013.11.3.