Ngày nhận bài: 27-12-2016 / Ngày duyệt đăng: 28-04-2017 / Ngày xuất bản: 06-08-2025
Bacterial leaf streak (BLS) disease in rice, caused by Xanthomonas oryzae pv. oryzicola (Xoc), has become a more common and serious disease in tropical regions along with bacterial leaf blight. Breeding new rice varieties by introducing resistance genes is considered one of the most effective and eco-friendly ways to control the disease. With a high diversity of resistance genes, local rice varieties play an important role in developing durable resistant varieties against diseases. In this study, 50 local rice accessions provided by Center for Conservation and Development of Crop Genetic Resources (CCD-CGR) were used to evaluate the reaction pattern with two isolates Xoc, TB4 and TN158. Lesion lengths measured after artificial inoculation showed 3 highly resistant (HR) accessions and 15 resistant (R) accessions to both TB4 and TN158 isolates. Four pairs of flanking SSR markers, RM587, RM510, RM153, and RM159, were used to detect the bls1 and qBlsr5a genes. The results of genotyping revealed 6 accessions containing the bls1 gene and 6 accessions containing qBlsr5a, of which, accession 11189 contained both genes. Based on the correlation analysis between reaction patterns and genotypes, the resistance ability of the bls1 gene was highly effective to Xoc, while the qBlsr5a gene did not show clear resistance. With our results, accessions that were HR or R, and carried the bls1 gene could be used as donors for breeding new rice varieties resistant to BLS.
