Date Received: 27-02-2023 / Date Accepted: 05-01-2024 / Date Published: 12-06-2025
This study was conducted with the aim of preparing chitosan nanocapsules transduced with trans-cinnamaldehyde for communication inhibition in bacteria. We used emulsification method to synthesize nanocapsule and nanoemulsion complexes. The nanocapsule and nanoemulsion complexes were transduced with trans-cinnamaldehyde using an oil nucleus and chitosan as cover. The nanocomplex had a uniform particle structure, low dispersion, and the size ranges from 120 to 150nm. Both nanocapsule and nanoemulsion were stable in bacterial culture after 24h of incubation. The trans-cinnamaldehyde transduction efficiency for the nanocapsule and nanoemulsion complexes was 86 and 73%, respectively. Both complexes released the drug through two stages, slowly in the first 6 hours and followed by continuous drug release after 12 hours, reaching 35 and 45%, respectively, for the nanocapsule and nanoemulsion. Bioassays with the E. coli Top 10 biosensor showed that nanocapsule was superior to nanoemulsions in inhibiting bacterial communication, also known as quorum sensing. In the best formulation, nanocapsule inhibited the communication process in this bacterium up to 65%.
