Establishment of a High-Efficiency Regeneration System for Petunia hybrida Hort. ex Vilm.-Andr. cv. ‘Midnight Gold’ from Leaf Tissues

Date Received: 08-02-2025

Date Published: 21-02-2025

##submissions.doi##: https://doi.org/10.1234/csv3yz47

Issue: Vol. 22 No. 11 (2024)

Section:

NÔNG HỌC

How to Cite:

Trang, P., Dung, N., Dao, T., & Ha, P. (2025). Establishment of a High-Efficiency Regeneration System for Petunia hybrida Hort. ex Vilm.-Andr. cv. ‘Midnight Gold’ from Leaf Tissues . Vietnam Journal of Agricultural Sciences, 22(11). https://doi.org/10.1234/csv3yz47

Establishment of a High-Efficiency Regeneration System for Petunia hybrida Hort. ex Vilm.-Andr. cv. ‘Midnight Gold’ from Leaf Tissues

Pham Thi Huyen Trang , Nguyen Thi Phuong Dung , Tran Thi Dao , Phung Thi Thu Ha (*)

  • Tác giả liên hệ: phungthithuha@vnua.edu.vn

    • Keywords

    Activated charcoal, BA, NAA, Petunia hybrida Hort. ex Vilm.-Andr. cv. ‘Midnight Gold’, regeneration system

    Abstract


    Black double petunia (Petunia hybrida Hort. ex Vilm.-Andr. cv. ‘Midnight Gold’), belonging to the Solanacea family, is a high - value and popular potted ornamental plant on the flower market. This study aimed to establish an efficient in vitro regeneration system for Petunia hybrida “Midnight Gold”. A one-factor experiment was arranged in a completely randomized design with three replications. Leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with various growth regulators and additives. The results showed that the optimal medium for shoot regeneration and multiplication was MS medium containing 1 mg/l benzyl adenine (BA) and 6.5 g/l agarose. For shoot development, MS medium with 30 g/l sucrose, 5% coconut water, and 6.5 g/l agarose was most effective for shoot tips, while 10% coconut water was preferred for nodal explants. Rooting was successfully induced on MS medium supplemented with 30 g/l sucrose, 0.1 g/l activated charcoal, and 6.5 g/l agarose. Acclimatized plantlets exhibited a 75% survival rate, reaching an average height of 7.83cm and producing 16.55 leaves per plant within four weeks. These findings provide a valuable protocol for Petunia hybrida “Midnight Gold” in vitro mass propagation, meeting the increasing market demand for this popular ornamental species.

    References

    Aguilar M.L., Espadas F., Maust B. & Sáenz L. (2009). Endogenous cytokinin contentin coconut palms affected by lethal yellowing. Journal of Plant Pathology. 91(1): 141-146.

    Borkird C. & Sink K.C. (1983). Medium Components for Shoot Cultures of Chlorophyll-Deficient Mutants of Petunia inflata. Plant Cell Reports. 2: 1-4.

    Bùi Thị Cúc, Đồng Huy Giới & Bùi Thị Thu Hương (2017). Nhân nhanh in vitro cây Dạ yến thảo hoa hồng sọc tím (Petunia hybrida L.). Tạp chí Khoa học và Công nghệ Lâm nghiệp. 10: 3-10.

    Farooq I., Qadri Z.A., Rather Z.A., Nazki I.T., Banday N., Rafiq S., Masoodi K.Z., Noureldeen A. & Mansoor S. (2021). Optimization of an improved, efficient and rapid in vitro micropropagation protocol for Petunia hybrida Vilm. cv. ‘‘Bravo”. Saudi Journal of Biological Sciences.

    : 3701-3709.

    Habas R.R., Turker M. & Ozdemir F.A. (2019). In vitro multiple shoot regeneration from Petunia hybrida. Turkish Journal of Agriculture - Food Science and Technology. 7(10): 1554-1560.

    Hassan A.Q., Anas A.R. & Sami Y. (2010). In vitro regeneration and somaclonal varition Petunia hybrida. Journal of Fruit and Ornamental Plant Research. 18 (1): 71-81.

    Lê Bảo Phúc, Võ Trang Anh Thư & Võ Thanh Phúc (2022). Ảnh hưởng của Nano bạc lên sự phát sinh chồi và ra rễ của cây Dạ yến thảo (Petunia Hybrida L.). Tạp chí Khoa học và Công nghệ - Đại học Đà Nẵng. 20(11.1): 13-18.

    Martineau B., Hanson M.R. & Ausubel F.M. (1981). Effect of charcoal and hormones on anther culture of Petunia and Nicotiana. Zeitschrift fur Pflanzenphysiologie. 102(2): 109-116.

    Murashige T. & Skoog F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Plant Physiology. 15: 473-497.

    Ndagijimana V., Kahia J., Asiimwe T., Sallah P.Y., Waweru B., Mushimiyimana I., Ndirigwe J., Kirimi S., Shumbusha D., Njenga P., Kouassi M., & Koffi E. (2014). In vitro effects of gibberellic acid and sucrose concentration on micropropagation of two elite sweet potato cultivars in Rwanda. International Journal of Biotechnology and Molecular Biology Research. 5(1):1-6.

    Nguyễn Tiến Long, Lã Thị Thu Hằng, Trần Thị Triêu Hà, Dương Thanh Thủy & Lê Như Cương (2021). Nghiên cứu tạo nguồn vật liệu khởi đầu trong nhân giống in vitro cây hoa Dạ yến thảo (Petunia hybrida L.). Tạp chí Khoa học và Công nghệ Việt Nam. 63 (7): 53-56.

    Nowak B., Miczyński K. & Hudy L. (2004). Sugar uptake and utilisation during adventitious bud differentiation on in vitro leaf explants of ‘Wegierka Zwykła’ Plum (Prunus domestica). Plant Cell, Tissue and Organ Culture.76: 255-260.

    Phạm Hoàng Hộ (2000). Cây cỏ Việt nam. Tập 2. Nhà xuất bản Trẻ. Tr. 769.

    Phạm Thị Huyền Trang, Nguyễn Thị Thúy Hạnh & Phùng Thị Thu Hà (2024). Nghiên cứu nhân in vitro Dạ yến thảo hoa tím (Petunia hybrida Hort.). Tạp chí Khoa học Nông nghiệp Việt Nam. 22(1): 1-9.

    Phan Xuân Huyên & NguyễnThị Phượng Hoàng (2017). Nghiên cứu tái sinh chồi và nuôi trồng cây Lan gấm (Anoectochilus formosanus Hayata). Tạp chí Công nghệ Sinh học, 15(3): 515-524.

    Thomas & Dennis T. (2008). The role of activated charcoal in plant tissue culture”, Biotechnol. Adva. 26: 618-631.

    Võ Thanh Phúc, Nguyễn Hoàng Huynh & Võ Thanh Truyền (2023). Thiết lập qui trình vi nhân giống hiệu quả cây Dạ yến thảo (Petunia hybrida Hort. ex Vilmor.). Tạp chí Khoa học và Công nghệ - Đại học Đà Nẵng. 21(9.1): 72-77.

    Yong J.W.H., Ge L., Yan F. Ng & Tan S.N. (2009). The chemical composition and biological properties of coconut (Cocos nucifera L.) water. Molecules. 14: 5144-5164.