Selecting Suitable Disinfection Method for Tissue Culture of Seaweed (Kappaphycus alvarezii)

Date Received: 08-02-2025

Date Published: 21-02-2025

##submissions.doi##: https://doi.org/10.1234/3vhwm882

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Issue: Vol. 22 No. 12 (2024)

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Mat, P., Tung, L., Duyet, N., & Nguyen, N. (2025). Selecting Suitable Disinfection Method for Tissue Culture of Seaweed (Kappaphycus alvarezii). Vietnam Journal of Agricultural Sciences, 22(12). https://doi.org/10.1234/3vhwm882

Selecting Suitable Disinfection Method for Tissue Culture of Seaweed (Kappaphycus alvarezii)

Pham Thi Mat (*) , Le Thanh Tung , Nguyen Thi Duyet , Nguyen Van Nguyen

  • Tác giả liên hệ: ptmat.rimf@gmail.com

    • Keywords

    Kappaphycus alvarezii, antibiotics, callus, silver nano, natri hypochlorite

    Abstract


    This study evaluated the effective method of disinfecting the sample surfaces at varying concentrations of bactericidal agents to produce axenic materials for Kappaphycus alvarezii micropropagation. The experiment was conducted with 3 types of bactericidal agents including (1) sodium hypochlorite solution at concentration of 0.25% for 5 seconds, (2) pure Sigma antibiotics at concentrations of 0.5%, 1% and 1.5% for 48 hours, (3) nano silver solution at concentrations from 100 to 1000 ppm for 5 minutes. The research results showed that using sodium hypochlorite solution to prevent bacterial contamination in K. alvarezii was the best disinfection method. The samples treated by sodium hypochlorite solution produced high rate of axenic explants (86.67%), highest rate of callus induction (94.44%) and good callus quality. For treatment of pure antibiotics and nano silver solution, when the concentration was higher, the axenic sample rate was higher but the survival rate and the callus induction rate were lower. The antibiotics with a concentration of 1.5% gave lowest callus rate (41.11%) and highest (54.44%) at a concentration of 0.5%. The concentration of nano silver solution at 1000 ppm gave the lowest callus rate (45.56%), while the 100 ppm formula had the highest callus rate (71.11%) but the lowest clean sample rate (only 12.22%).

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