A MYXOBACTERIUM STRAIN ISOLATED IN VIETNAM PRODUCES EREMOPHILENE-LIKE SESQUITERPENE

Ngày nhận bài: 10-09-2017

Ngày duyệt đăng: 10-10-2017

Ngày xuất bản: 06-08-2025

DOI: https://doi.org/10.31817/tckhnnvn.2017.15.9.

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Số: Tập 15 Số 9 (2017)

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KỸ THUẬT VÀ CÔNG NGHỆ

Cách trích dẫn:

Thuy, L., Nga, L., & Bach, N. (2025). A MYXOBACTERIUM STRAIN ISOLATED IN VIETNAM PRODUCES EREMOPHILENE-LIKE SESQUITERPENE . Tạp Chí Khoa học Nông nghiệp Việt Nam, 15(9), 1247–1255. https://doi.org/10.31817/tckhnnvn.2017.15.9.

A MYXOBACTERIUM STRAIN ISOLATED IN VIETNAM PRODUCES EREMOPHILENE-LIKE SESQUITERPENE

Ly Thi Bich Thuy 1, 2 , Le Thi Thien Nga 1, 2 , Nguyen Duc Bach (*) 1, 2

  • Tác giả liên hệ: [email protected]
  • 1 Institute of Biotechnology, Vietnam Academy of Science and Technology
  • 2 Faculty of Biotechnology, Vietnam National University of Agriculture

    • Từ khóa

    16S rRNA, eremophilene, Myxobacteria, sesquiterpene, secondary metabolites

    Tóm tắt


    Sesquiterpenes, a class of terpenes, consist of three isoprene units, constituting a highly diverse class of natural bioactive compounds that are found principally in plants but also in fungi and some invertebrates and myxobacteria as well. In this study, by using different approaches for isolation, analysis of morphology and bio-physio-chemical properties and comparison of the sequences of 16S ribosomal RNA genes, three strains belonging to the group of myxobacteria were isolated from soil samples collected from some areas in Vietnam. The sesquiterpene biosynthesis in the isolated strains was investigated. The data showed that the isolated strain DL1 was able to form fruiting body and sysnthesize an eremophilene-like sesquiterpene.

    Tài liệu tham khảo

    Alexander S., Ly T.B.T., Nils G., Zapp J., Thiel V., Schulz S., Hannemann F., Khatri Y. and Bernhardt R. (2015). Characterization of the gene cluster CYP264B1-geoA from Sorangium cellulosum So ce56: Biosynthesis of (+)-Eremophilene and its hydroxylation. ChemBioChem, 16: 337-344.

    Bártíková H., Hanusová V., Skálová L., Ambroz M. and Bousová I. (2014). Antioxidant, pro-oxidant and other biological activities of sesquiterpenes. Current Topics in Medicinal Chemistry, 14: 2478-2494.

    Gerth K., Pradella S., Perlova O., Beyer S., Müller R. (2003). Myxobacteria: proficient producers of novel natural products with various biological activities-past and future biotechnological aspects with the focus on the genus Sorangium. J Biotechnol, 106(2-3): 233 -53.

    Gram H.C. (1884). “Über die isolierte Färbung der Schizomyceten in Schnitt- und Trockenpräparaten” (in German). Fortschritte der Medizin, 2: 185-189.

    Hyesook H., Chung J., Kim J., Lee J.S., Kwon B.M., Son K.H, and Cho K. (2008). Isolation of Sorangium cellulosum carrying epothilone gene clusters. J. Microbiol. Biotechnol, 18(8): 1416-1422.

    Hyun H., Chung J., Kim J., Lee J.S., Kwon B.M., Son K.H., Cho K. (2008). Isolation of Sorangium cellulosum carrying epothilone gene clusters. J Microbiol Biotechnol, 18(8): 1416-1422.

    Konstantinidis K. T., Tiedje J. M. (2005). Genomic insights that advance the species definition for prokaryotes. Proc. Natl Acad. Sci. USA,

    : 2567-2572.

    Li S.G., Zhao L., Han K., Li P.F., Li Z.F., Hu W., Liu H., Wu Z.H., Li Y.Z. (2013). Diversity of epothilone producers among Sorangium strains in producer-positive soil habitats. Microb Biotechnol, 7(2): 130-141.

    Ly T.B.T., Alexander S., Nguyen D.B., Bernhardt R. (2017). Improvement of a P450-based recombinant E. coli whole-cell system for the production of oxygenated sesquiterpene derivatives. J. Agric. Food Chem, doi: 10.1021/acs.jafc.7b00792.

    Nguyen N.P., Warnow T., Pop M., White B. (2016). A perspective on 16S rRNA operational taxonomic unit clustering using sequence similarity. NPJ Biofilms Microbiomes, 2: 16004.

    Reichenbach H. (1983). A simple method for the purification of myxobacteria. J Microbiol Methods, 1: 77-79.

    Reichenbach H. (1999). The ecology of the myxobacteria. Environ.Microbiol, 1: 15-21.

    Reichenbach H. and Dworkin M. (1986). The myxobacteria. The Prokaryotes 4. Springer,

    Third Edition.

    Schneiker S., Perlova O., Kaiser O., Gerth K., Alici A., Altmeyer M.O., Bartels D., Bekel T., Beyer S., Bode E., Bode H.B., Bolten C.J., Choudhuri J.V., Doss S., Elnakady Y.A., Frank B., Gaigalat L., Goesmann A., Groeger C., Gross F., Jelsbak L., Jelsbak L., Kalinowski J., Kegler C., Knauber T., Konietzny S., Kopp M., Krause L., Krug D., Linke B., Mahmud T., Martinez-Arias R., McHardy A.C., Merai M., Meyer F., Mormann S., Munoz-Dorado J., Perez J., Pradella S., Rachid S., Raddatz G., Rosenau F., Rückert C., Sasse F., Scharfe M., Schuster S.C., Suen G., Treuner-Lange A., Velicer G.J., Vorhölter F.J., Weissman K.J., Welch R.D., Wenzel S.C., Whitworth D.E., Wilhelm S., Wittmann C., Blöcker H., Pühler A. and Müller R. (2007). Complete genome sequence of the myxobacterium Sorangium cellulosum. Nat Biotech, 25:1281-1289.

    Shimkets L.J., Dworkin M., and Reichenbach H. (2006). The myxobacteria. In The Prokaryotes. E.(eds). New York: Springer, pp. 31-115.

    Wenzel S.C. and Müller R. (2007) Myxobacterial natural product assembly lines: fascinating examples of curious biochemistry. Nat Prod Rep, 24: 1211-1224.

    Wilson K. (2001). Preparation of genomic DNA from bacteria. Curr Protoc Mol Biol, doi: 10.1002/0471142727.mb0204s56.

    Wu Z.H,, Jiang D.M., Li P., Li Y.Z. (2005). Exploring the diversity of myxobacteria in a soil niche by myxobacteria-specific primers and probes. Environ Microbiol, 7(10): 1602-1610.