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• Genetic Structures of Estrogen Receptor (ESR) and Prolactin Receptor (PRLR) Genes in Landrace and Yorkshire Populations at DABACO Nucleus Pig Breeding Company

  Nguyen Chi Thanh, Tran Xuan Manh, Nguyen Van Hung, Luu Thi Trang, Phan Xuan Hao, Vu Dinh Ton

  Date Received: 02-05-2019 / Date Accepted: 30-09-2019

   Abstract PDF (VIETNAMESE)

  Litter size of pigs is an important economic trait, but its heritability is relatively low,at approximately 0.1. In addition, litter size trait can only be measured on female pigs at mature age. Therefore, genetic improvement by phenotypic selection is less effective, and at slow rate. In previous literature, it is found that ESR and PRLR genes are two QTLswith large effects on litter size in pigs. Of the two alleles A and B at ESR locus, allele B shows a positive effect on the litter size trait,while of the two alleles A and B at PRLR locus, allele A is benefical to the trait. Landrace and Yorkshire, which are imported, are two major breeds in Vietnam. The current study aimed to evaluate genetic structures of ESR and PRLR genes in two pig populations of Landrace and Yorkshire reared at the Dabaco nuclear breeding pig company, and thus examine the effects of ESR and PRLR genes on reproduction traits of the two pig populations. It was found that all three genotypes,AA, AB and BB at ESR and PRLR loci,were present in both the populations of Landrace and Yorkshire pigs. At ESR locus, allele frequency of B in the Landrace population (0.184) was lower than in the Yorkshire population (0.413). The frequency of homozygous genotype BB was 0.041 for the Landrace population and 0.655 for the Yorkshire population. At PRLR locus, allele frequencies of A and B were quite similar for the two populations. Allele frequency of A was 0.483 for Landrance and 0.516 for Yorkshire. Allele frequency of B was 0.517 for Landrance and 0.484 for Yorkshire.

• Effect of Salinity on Reproductive Performance of SelectedBrackish Water NileTilapia Strain (Oreochromis niloticus)

  Le Minh Toan, Vu Van Sang, Trinh Dinh Khuyen

  Date Received: 27-09-2012 / Date Accepted: 18-12-2012

   Abstract PDF (VIETNAMESE)

  The experiment was carried out to evaluate the effects of salinity on fertility of selected brackish water Nile tilapia (Oreochromis niloticus) strain in 53 days. The experiment fish was arranged breeding in brackish water corresponding to three different salinity levels such as: 4 - 5‰, 8 - 10‰ and 14 - 16‰, the ratio of male: female tilapia was 1:2, each formula was repeated 3 times in 3 different tanks, stocking density was 4 individuals/m3 for each tank. Results show that the reproductive rate of females corresponding to 4 - 5‰ salinity was 26.2 ± 4.64%; 8 - 10‰: 17.86 ± 11.21% and 14 - 16‰: 6.88 ± 6.57%. The number of eggs per formula obtained after 7 times was obtained in 3 corresponding levels salinity was 5,229a ± 1,280 eggs, 2,721b ± 1,559 eggs and 923c ± 706 results. Relative fecundity 3 levels of salinity 4 - 5‰, 8 - 10‰ and 14 - 16‰, respectively, 4.3a ± 0.63 eggs/g fish/time was 3.36b ± 0.67 eggs/g female/times and 1.7c ± 1.27 eggs/g fish/time. Absolute fecundity of tilapia in salinity 4 - 5% was 829 ± 122.9 eggs/time delivery, salinity 8 - 10‰ of 678a ± 135,7 and at a salinity of 14 - 16‰ was 403b ± 154.5 eggs/female/delivery time. Natural frequency of females at 4 - 5‰ salinity 22.3 ± 2.23 days; 8 - 10% was 24.9 ± 3.7 days and 14 - 16‰ salinity was 25.7 ± 2.59 days. Experimental results showed that the breeding of tilapia at salinity level of 4 - 5‰ obtained the best reproduction, next to the level of salinity 8 - 10‰. Experiments show that the breeding of Nile tilapia at salinity level below 8 - 10‰ was high practical significance.

• Investigation of Genetic Variation of Some Stocks of White Leg Shrimp (Litopenaeus vannamei) Cultured in Vietnam Using Microsatellite Markers

  Tran Thi Thuy Ha, Nguyen The Viet, Nguyen Thi Huong, Nguyen Huu Duc

  Date Received: 29-05-2013 / Date Accepted: 20-08-2013 / Date Published: 02-07-2025

   Abstract PDF (VIETNAMESE)

  In this study, five microsatellite loci in two sets of primers pooled PCR were used to assess the genetic diversity of five populations of white leg shrimp (Litopenaeus vannamei) cultured in Vietnam. The number of alleles per locus ranges from 6 to 8 and low alleles frequency is observed at all loci (<0.1). The ​​observed heterozygosity (Ho) ranges from 0.21 to 0.50,  lower than the expected heterozygosity (He, 0.41 - 0.72). The average Fis value varies from 0,18 to 0.40, and high value found at loci TUMXL v6.23 and Lvan 01) for all populations, 0.446 and 0.496, respectively. Fst values were in ​​average (0.054 – 0.185) and no significant genetic differences among five populations. The finding shows the evidence of heterozygote deficiencies all over five populations leading to a question for consideration of a solution. The selective breeding program of white leg shrimp should be done for sustainable development of the species.

  DOI: https://doi.org/10.31817/tckhnnvn.2013.11.6.

• Genetic Association between Single Nucleotide Polymorphisms of PIT-1 Gene and Milk Production in Holstein Friesian Cows

  Nguyen Thi Thu, Nguyen Thi Minh Hang, Nguyen Huu Duc, Ha Thanh Tùng, Le Thi Chau, Le Van Ty, Nguyen Thi Dieu Thuy

  Date Received: 17-05-2013 / Date Accepted: 25-08-2013 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  PIT-1, a regulatory transcription factor, plays a role in pituitary cell differentiation and proliferation and expression level of hormones in the anterior pituitary gland. The objective of present study was to identify the single nucleotide polymorphisms of PIT-1 gene and their association with milk yield in Holstein Friesian (HF) cows. Milk yield was recorded for the whole lactation period. PCR-RFLP technique was applied for genotyping of two SNPs belonging to exon 2 (E2) and exon 6 (E6) with restriction enzymes TaqI and HinfI. The analysis of 53 Holstein milking cows showed that genotypic frequencies of AA, AB and BB of PIT-1E2 were 45.3, 28.3 and 26.4%, respectively. For the PIT-1E6, the highest frequency of GG genotype (73.6%), the lowest frequency of genotype TT (7.5%) and TG genotype (18.9%) were found. Minitab software was used to analyze genotype - phenotype association. The result revealed that theere exists positive association between locus PIT-1E2 with milk yield (P= 0.037 and ρ = 0.28) and number of milking day (P=0.015 and r= 0.306), whereas, no association of PIT-1E6 was found (P>0.05). Cows with AA genotype yielded more milk than those with BB and AB genotypes. These finding showed the potential use of AA genotype of PIT-1E2 in assisting breeding for increased milk production of Holstein Friesian cows.

  DOI: https://doi.org/10.31817/tckhnnvn.2013.11.6.