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• Effects of Calcium Supplement to Water on Growth, Survival Rate and Productivity of Apple Black Snail (Pila polita)in Nursing Phase

  Le Minh Tue, Nguyen Thi Thanh Thuy, Ha Thi Hue

  Date Received: 26-09-2023 / Date Accepted: 12-06-2024 / Date Published: 12-06-2025

   Abstract PDF (VIETNAMESE)

  This research was conducted to evaluate the effect of different calcium concentrations on the growth of black apple smail in the nursing phase. The experiments were designed with four treatments and three replicates as follow: treatment 1 with 5mg Ca2+mg/l, treatment 2 with 10mg Ca2+mg/l, treatment 3 with 15mg Ca2+mg/l and the control (0mg Ca2+). The supplement of calcium was practiced every seven days. After 40 days, treatment 2 reached the highest weight, height, and body width with 2.22g; 20.75mm; 17.35mm, respectively, but statictially non-significance between treatments (P >0.05). The survival rate of snails also reached the highest value with treatment 2 at 87.3%, treatment 1 at 82.1%, treatment 3 at 78.3% and the lowest at 0% with control treatment. Similarly, the productivity of black apple snails was recorded the highest in treatment 2 at 4.04 g/m2, treatment 3 at 3.66 g/m2, treatment 1 at 2.02 g/m2and the lowest for the control at 0 g/m2.

  DOI: https://doi.org/10.31817/tckhnnvn.2024.22.6.

• Effect of Calcium and Salicylic Acid on Growth of Yardlong Bean under Salt Stress Condition

  Nguyen Thi Phuong Dung, Tran Anh Tuan

  Date Received: 20-02-2017 / Date Accepted: 19-06-2017

   Abstract PDF (VIETNAMESE)

  The aim of this study was to determine the effect of exogenous application of salicylic acid (SA 0.25 mM) and calcium nitrate (Ca(NO3)250 mM), singly or in combination, on plant growth, photosynthetic capacity, and some stress markers of yardlong bean plants exposed to salt stress (0.3% NaCl). Application of 0.3% NaCl reduced plant growth, total chlorophylland carotenoid content, and chlorophyll fluorescent (Fv/Fm). Exogenous application of SA or Ca2+alone alleviated the level of proline content, saturation of water shortage, hydrogen peroxide (H2O2), ion leakeageandmalondialdehyde (MDA) in the presence of NaCl. Nevertheless, the best results in growth of stemsand leaves, photosynthetic pigment concentrations, and some stress markers were observedin response to the combined SA + Ca2+treatment, except accumulation of dry matterand ion leakeage. Our results suggestedthat Ca2+and SA may interact to reduce the stress experienced by the plantsin the presence of NaCl.

• Calcineurin B-Like Protein Cbl4 and Cbl5 Participate in SignalTransductioninGerminating Rice (Oryza sativaL.) under Water Submergence

  Ho Viet The, Pierdomenico Perata, Chiara Pucciariello

  Date Received: 25-07-2014 / Date Accepted: 09-10-2015 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  OsCIPK15is a key regulator of starch degradation in rice due to submergence. This gene receives submergence signal via calcineurin B-like (OsCBL) proteins, which act as the Ca2+sensors, and whose role has yet to be established. In the present study, we compared the transcriptional regulation of CBLsin germinating rice embryos under water submergence. The trans-activation of OsCBLpromoters was tested by examining the effects of Ca2+. We used rice protoplasts to study the OsCBLs-OsCIPK15 protein interactions. We found that OsCBL4and OsCBL5were transcriptionally regulated under submergence.The Ca2+sensors OsCBL4 and OsCBL5 wereinvolved in this system and interactedon the plasma-membrane with OsCIPK15 in Ca2+dependent manner. Our results suggest that Ca2+signaling formed under submergence are inducedby OsCBL4and OsCBL5 which then interact with OsCIPK15 to activate genes involving instarch catabolism to provide energy for rice germination.

  DOI: https://doi.org/10.31817/tckhnnvn.2015.13.7.

• In VitroEffects of Minerals on Sporulation and Heat Resistance of BacillussubtilisCM3.1

  Pham Thi Tuyet Ngan, Vu Hung Hai, Huynh Truong Giang

  Date Received: 29-03-2023 / Date Accepted: 05-10-2023

   Abstract PDF (VIETNAMESE)

  This study sought to evaluate the in vitro effects of minerals, including calcium (Ca2+),potassium (K⁺), manganese (Mn2+),magnesium (Mg2+) and iron(Fe2+) on sporulation efficiency and heat resistance of BacillussubtilisCM3.1. Sporulation of Bacillus cells was induced in sporulation medium supplemented with various concentrations of the minerals. 7 days after inoculation, Ca2+(1mm), Mg2+(3mm), Mn2+(1mm) and Fe2+(1mm) as single supplements into Nutrient Broth (NB) significantly increased spore cell density of BacillussubtilisCM3.1, except for K+(10mm). Furthermore, the spore cell density and viability of Bacillus were detectable in sporulation media such as m-NB, SSM and m-SM, significantly higher compared to those in NB (P <0.05) after heating periods, especially from 30 to 40 minutes of exposure. Our results could provide better understanding of the importance of minerals supplemented into culture medium in improving the density, heat resistance of Bacillus spore, and thus enhancingthe stability of probiotic Bacillus subtilis CM3.1 during manufacturing process and storage time.

• Analysis of Blood Haematological and Biochemical Parameters in Buffaloes (Bubalus bubalis) Raised in Cam Thuy District, Thanh Hoa Province

  Nguyen Ba Tiep, Luu Tuan Nghia

  Date Received: 09-06-2016 / Date Accepted: 23-11-2016 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  This study was done to determine blood haematological parameters (red blood cell count, hemoglobin, white blood cell count, white blood cell composition, platelet cell count and other platelet parameters) and blood biochemistry (total plasma proteins, serum albumin, globulin and glucose, serum sodium, serum postassium, serum chloride and calcium levels) of water buffaloes raised in Cam Thuy district, Thanh Hoa province. Red blood cell count, hemoglobin values, white blood cell count, platelet cell count, plasma total protein and globulin concentrations were age-dependent. Sex dependences were observed in red cell distribution width but not in white blood cell and platelet values. The determined values were compared with reference values of the International Federation of Clinical Chemistry (IFCC) and results obtained from previous studies. The present results can be employed as reference data for study, diagnosis and treatment of bufalo diseases with widely used automated analyzers for clinical blood parameters.

  DOI: https://doi.org/10.31817/tckhnnvn.2016.14.11.

• Purification and Characterization of Cellulase from Bacillussp. M5Strain

  Trinh Thi Thu Thuy, Nguyen Thi Thanh Thuy, Nguyen Thi Lam Doan, Hoang Thi Ngoc

  Date Received: 20-06-2018 / Date Accepted: 28-11-2018

   Abstract PDF (VIETNAMESE)

  The objective of the present study was to purify and determine the properties (optimal temperature, heat stability, optimal pH, pH stability, effects of metal ions) of cellulase derived from Bacillussp. M5strain. The crude enzyme produced from the culture of cellulase-producing bacillus M5 strain was collected by centrifugationand precipitated for purification andcharacterization. To investigate the enzyme's characteristics onoptimal temperature, pH as well as heat stability, pH stability and the effect of certain metal ions, the enzyme fluid was treated under different conditions. The enzymewas disolvedin acetate buffer pH 5.50 mM to determine enzyme activity. Results showed that the cellulase from Bacillus M5 strain was active at an optimaltemperature of 65C; optimalpH of 5.5; heat stability at about 50C (activity remains53% after 120 minutes); pH of 6.0 to 6.5.Ca2+and Mg2+metal ions increasedenzyme activity and Zn2+ion decreasedenzyme activity. The enzyme hadthemolecular weight of 45 kDa.

• Effect of Ecological Zones of Fresh and Brackish Water on Rice Growth and Soil Chemical Characteristics in Rice-Shrimp System in Bac Lieu Province

  Le Van Dang, Ngo Ngoc Hung

  Date Received: 18-04-2018 / Date Accepted: 12-07-2018

   Abstract PDF (VIETNAMESE)

  The objectives of the study were to evaluate i) effect of ecological zones of fresh and brackish water on rice-shrimp cultivation systems, (ii) chemical characteristics of soil and water of the agro-ecological zones, and (iii) the efficiency of liming on rice growth and yield. Field experiments were conducted during August to December 2013 in Phuoc Long and Hong Dan, Bac Lieu province. Results showed that, after shrimp harvest, Phuoc Long planted rice crop in August when soil salt concentration was high (ECe: 8.0 mS/cm; ESP: 13.8%). Exchange cations in the soil decreased in the descending order: Mg2+>Na+>Ca2+>K+, corresponding to 8.0>3.0>1.5>1.0 meq/100 g, respectively. Due to salt accumulation in the soil in brackish water zone, ECe and ESP were at high levels during December (5,6 mS/cm and 7,8%, respectively), unfavourable for growth and yield of rice in comparison with fresh water zone at Hong Dan. Lime application to the salt-affected soil in Phuoc Long reduced salt damage to rice in terms of panicle number per square meter, filled grain rate, and higher grain yield (by 0.6 t ha-1) as compared to without liming. However, lime application  to rice soil in fresh water zone at Hong Dan did not significantly increase the grain yield.