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• RAPD and ISSR Markers Assessment of Genetic Diversity of 12 Cordyceps Accessions collected in Vietnam

  Nong Thi Hue, Tran Thi Thao, Le Phuong Linh, Ninh Thi Thao, Ngo Xuan Nghien, Dinh Truong Son

  Date Received: 15-08-2023 / Date Accepted: 25-12-2023

   Abstract PDF (VIETNAMESE)

  Cordyceps spp. belong to the most valuable medicinal caterpillar fungi. However, consumers hardly know the origin and species of Cordycepsspp. commercialized on the market. This study aimed to identify 12 collected Cordyceps samples collected at various locations in Vietnam by ITS sequencing and evaluate the genetic diversity these sampless using RAPD and ISSR markers. 12 Cordyceps accessions were identified as Cordyceps militarisusingITS sequencing. 15 RAPD markers and 15 ISSR markers successfully detected 340 loci of which 291 were polymorphic and a total of 2092 scorable bands were obtained.The similarity coefficientof 12 Cordyceps spp. accessionsrangedfrom 0.568 to 0.841 when combined RAPD and ISSR markers, indicating high genetic diversity. At a genetic similarity of 0.7, the UPGMA dendrogram constructed based on RAPD and ISSR makers separated 12 Cordyceps accessions into three distinct clusters. The correlation analysis between the matrices of similarity coefficients using Mantel’s test revealed a strong correlation among matrices analyzed by RAPD, ISSR or pooled data. Our results could provide useful information for the exploitation and breeding of Cordyceps militarisaccessions.

• The Preliminary Investigationon Cultivation Technology of Orange Caterpillar Fungus Cordyceps militaris L.ex Fr. in VietNam

  Le Van Ve, Tran Thu Ha, Nguyen Thi Bich Thuy, Ngo Xuan Nghien

  Date Received: 18-12-2014 / Date Accepted: 21-04-2015

   Abstract PDF (VIETNAMESE)

  Four fruiting media(MT-1, MT-2, MT-3 and MT4) were used to cultivate Cordycepsmilitaris(orange caterpillar fungus). Results showed that MT-4 had highest biological productivity(11.63± 1.34%).Among four basal substrates including three brown rice typesA, B, C and paddy riceD were assessed. With biological productivity of 10.92± 1.96%, type B brown rice considered asthe most suitable substrateto cultivate orange caterpillar fungus. However, fifth subculturestarted to showy degenerationof fungal stock as evidenced by the change offruting-body colour, the mycelial density,the number of fruiting-body primordia andbiological productivity.

• Identification of the Mating-type Loci of Cordyceps militarisby Multiplex PCR

  Nguyen Minh Ly, Vo Ba Duy, Dinh Xuan Tu

  Date Received: 01-03-2023 / Date Accepted: 05-10-2023

   Abstract PDF (VIETNAMESE)

  In the cross-breeding of Cordyceps militaris using single spores, determining the genotype of the mating type locus is crucial for constructing a cross diagram. Individual PCRshave been conducted to identify the mating type loci MAT1-1 and MAT1-2 in this fungus. The research aimed to design specific primers for Multiplex PCR to efficiently and simply identify mating-type loci in a shorter time. The primer sequences were derived from the sequences of two mating type loci MAT1-1 (AB194982) and MAT1-2 (AB084257) retrieved from the GenBank database using the Primer-BLAST tool. The research results indicate that the Multiplex PCR, comprising three primer pairs MAT1-1-1-MUL, MAT1-1-2-MUL, and MAT1-2-1-MUL, successfully amplified products with sizes of 527bp, 323bp, and 222bp from the MAT1-1-1, MAT1-1-2, and MAT1-2-1 genes, respectively. The Multiplex reaction exhibits high specificity and reliability in determining mating genotypes in C. militaris, making it a valuable tool for applications in breeding selection based on molecular markers in C. militaris.