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• Preliminary Results on Transfer of Frozen Gender-separated Embryos in Cattle of TH Milk Joint Stock Company in Nghia Dan, Nghe An

  Pham Tuan Hiep, Ha Dinh Hieu, Le Van Thien, Hoang Kim Giao

  Date Received: 18-04-2017 / Date Accepted: 26-07-2017 / Date Published: 12-06-2025

   Abstract PDF (VIETNAMESE)

  From December, 2015 to December, 2016, for the first time 1,117 frozen gender-separated embryos were imported to Vietnam. In the cooperation program between TH milk group and Sexing Technology Companythe embryos weretransferred to aherd of heifers from 13 - 16 months old in TH’s Farm in Nghia Dan, Nghe An. Total 2143 heifers were synchronized for estrus by PGF2α. 1,661/2,143 = 77.5%%, in which 1,035 /1,637= 63.2% heifers were qualifiedfor receivingembryo. 1,051 gender separated embryos were transferred to1,051 heifers (in which 1,035 estrous heifers by injection PGF2α and 16 heifers naturally estrous). After 35 days of embryo transplantation(42 days age of embryo) the pregnancy was checked by Ultrasound or by rectal palpation. 488/1,051 = 46.4% heifers became pregnant, the pregnancy rate depending onseason, farmcondition, quality and location of corpus luteum on heifer receivingembryos, and skillsof technicians. The highest pregnancy rate was52.6%and thelowest was 40.9%. During the pregnancy, 10% of pregnant heifers showed abortionand5% with stillborn. The pregnant heifers gave birth to203 normal calves, including195 female calves (96.05%)and 8 male calves (3.95%).

  DOI: https://doi.org/10.31817/tckhnnvn.2017.15.7.

• Study on The Transfer of Auxin Susceptible Gene (Ino-Rolb) into Duong Canh Mandarin and Vinh Orange

  Phan Huu Ton, Tong Van Hai, Doan Van Lu, Nguyen Duc Bach, Abhaya Dandenkar

  Date Received: 11-07-2013 / Date Accepted: 28-09-2013 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  The apomixis phenomenon in citrus often leads to small, seedless and poor quality fruits. It is due to no or very less auxin is synthesized in the ovary leading to the abnormal development of embryo. Therefore, the fruits can not develop normally. Of many different strategies for seedless breeding in citrus such as outcrossing, triploidy, mutagenesis, the transfer of auxin-sensitive gene particularly expressed in ovary is a very useful approach. In this study, the vector pDU04.4522 carrying the auxin-sensitive gene (INO-rolB) working under the control of INO promoter was transferred into Duong Canh mandarine and Vinh orange. In order to identify the optimal conditions for gene transfer, four experiments focusing on the co-culture stage were carried out. Results showed that the auxin-sensitive gene INO-rolB was sucessfully transferred into the cotyledon of  Duong Canh tangerine and Vinh orange. The optimal conditions for  high efficiency of gene transfer in co-culture medium were at 22°C for 3 days in the presence of 50 - 100mM acetosyringone and 2 mg/l NAA.

  DOI: https://doi.org/10.31817/tckhnnvn.2013.11.6.

• Challenges and Opportunities in Implementation of Reproductive Technologies for Livestock Industry in Vietnam

  Bui Xuan Nguyen, Nguyen Thi Uoc, Duong Dinh Long, Phan Ngoc Minh, Nguyen Khac Tich

  Date Received: 18-04-2017 / Date Accepted: 26-07-2017 / Date Published: 12-06-2025

   Abstract PDF (VIETNAMESE)

  The growing of world population and the increasing demand for meat and milk in the coming decades are posing challenges for the development of livestock industry in developing countries, including Vietnam. In this article we l briefly outlinedthe actual trends of application of reproductive technologies for the development of livestock industry in the 21 century,the current status of research and application of embryo transfer, in vitrofertilization, embryo sexing, cell and embryo cryopreservation and somatic cloning technique for both aspects of animal breeding and animal biodiversity conservation in Vietnam. By evaluating and analyzing the limitations of technology application due to the conditions of small-scale animal husbandry, the authors discussedthe necessary strategy for the further successful implementation of reproductive technologies.

  DOI: https://doi.org/10.31817/tckhnnvn.2017.15.7.