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RAPD and ISSR Markers Assessment of Genetic Diversity of 12 Cordyceps Accessions collected in Vietnam

Nong Thi Hue, Tran Thi Thao, Le Phuong Linh, Ninh Thi Thao, Ngo Xuan Nghien, Dinh Truong Son

Date Received: 15-08-2023 / Date Accepted: 25-12-2023
Cordyceps spp. belong to the most valuable medicinal caterpillar fungi. However, consumers hardly know the origin and species of Cordycepsspp. commercialized on the market. This study aimed to identify 12 collected Cordyceps samples collected at various locations in Vietnam by ITS sequencing and evaluate the genetic diversity these sampless using RAPD and ISSR markers. 12 Cordyceps accessions were identified as Cordyceps militarisusingITS sequencing. 15 RAPD markers and 15 ISSR markers successfully detected 340 loci of which 291 were polymorphic and a total of 2092 scorable bands were obtained.The similarity coefficientof 12 Cordyceps spp. accessionsrangedfrom 0.568 to 0.841 when combined RAPD and ISSR markers, indicating high genetic diversity. At a genetic similarity of 0.7, the UPGMA dendrogram constructed based on RAPD and ISSR makers separated 12 Cordyceps accessions into three distinct clusters. The correlation analysis between the matrices of similarity coefficients using Mantel’s test revealed a strong correlation among matrices analyzed by RAPD, ISSR or pooled data. Our results could provide useful information for the exploitation and breeding of Cordyceps militarisaccessions.
Genetic diversity analysis of 30 accessions of lotus (Nelumbo spp.) by RADP and ISSR markers

Ngo Thi Hong Tuoi, Pham Thi Ngoc, Nguyen Thi Bich Hong, Ha Thi Loi, Nguyen Thi Hue, Pham Van Cuong, Dinh Truong Son

Date Received: 09-12-2022 / Date Accepted: 22-05-2023
Lotus (Nelumbo spp., Nelumbonaceace family) is an important horticultural plant, with its uses ranging from ornamental, nutritional to medicinal values, and has been widely used in Vietnam. This study aimed to evaluate the genetic diversity of 30 lotus accessions collected in some provinces in Vietnam by using RAPD and ISSR markers. 12 RAPD and 18 ISSR successfully detected 178 loci, among these, 115 were polymorphic loci and 4084 clear and scorable bands were obtained. The similarity coefficient of 30 accessions ranged from 0.65 to 0.93 when combined both RAPD and ISSR markers. At the genetic similarity of 0.80, 30 lotus accessions could be divided into 4 distinct clusters. These results showed that the genetic diversity of 30 collected lotus accessions were very high. The 30 collected accessions are valuable source for the conservation and development of lotus genetic resources as well as for the breeding of new lotus varieties.
Genetic Diversity Among 25 Accessions of Camellia spp. Collected in Quang Ninh by RADP and ISSR markers

Dang Quang Bich, Nguyen Thi Phuong Thao, Nguyen Van Phu, Ninh Thi Thao, Hoang Hai Ha, Doan Thi Thu Ha, Nguyen Thi Thuy Linh, Dinh Truong Son

Date Received: 24-05-2017 / Date Accepted: 19-09-2017 / Date Published: 06-08-2025
Golden flower tea plant (Camellia spp.) is one of the important medicinal plant. Genetic diversity among 25 accessions of Camellia spp., collected mostly in Quang Ninh, were evaluated by RAPD and ISSR molecular markers. Using 53 molecular markers (45 RAPD and 8 ISSR marker), we detected 294 loci with total 4656 clear and scorable bands, among these, 3628 were polymorphic bands. Based on a total of 4656 bands and similarity coefficient ranged from 0.54 to 0.81, dendrogram analysis was performed. With the genetic similarity at 0.71, 25 Camellia spp. accessions could be divided in to 9 distinct clusters. The combination of phenotypic analysis, genetic variation as well as analysis of pharmacologically active compounds showed that amongs 25 golden flower tea accessions, although they look similar, however, quite diverse in genotype and the ability to accumulate pharmacological compounds. The collected accession will be a valuable source for breeding programs of golden flower tea plants in Quang Ninh.
DOI: https://doi.org/10.31817/tckhnnvn.2017.15.8.
Analysis of Genetic Diversity of Citrus Genotypes Collected in Ha Giangby RAPD and ISSR Markers

Nguyen Thi Phuong Thao, Vu Van Hieu, Nong Thi Hue, Nguyen Thi Oanh, Ninh Thi Thao, Vu Quang Sang

Date Received: 24-03-2015 / Date Accepted: 15-09-2015 / Date Published: 13-06-2025
Oranges grown in Ha Giang areone of the most common citrus products in northern Viet Nam. However,vegetative propagation and selection for adaptation to various growing environments led to deviation from original genotype.Assessinggenetic diversity of indigenous citrus maycontribute to the collection, classification, evaluation and conservation as well as provide genetic information among citrus germplasm, which could be used for the breeding program. RAPD and ISSR analysis combined with the construction of phylogenetic tree revealed the genetic relationship among researched citruses. The results showed that 25 RAPD and 5 ISSR primers generated polymorphic patterns of PCR products (100%), however,the numbers of polymorphic bands detected on each genotype were significant in range. A total of 2157 DNA bands were foundwith an average of 1.80 DNA bands per genotype. RAPD markers (OPAW19andOPAE15)and ISSR markers (T1andT5)yielded highest polymorphism, with 4.48, 4.23 and 3.25, 3.45 bandsper genotype, respectively. The resultsalso indicated that the similarity coefficient of 39 samples ranged from 0.33 to 0.98 and 5 genetic groups were obtained.
DOI: https://doi.org/10.31817/tckhnnvn.2015.13.6.
Genetic Diversity Analysis of 10 Oyster Mushrooms (Pleurotusspp.) Accessions by RAPD and ISSR Markers

Dinh Truong Son, Nguyen Thi Ngoc Anh, Nguyen Thu Hang, Nguyen Thi Bich Thuy, Nong Thi Hue, Ngo Xuan Nghien

Date Received: 15-08-2023 / Date Accepted: 25-12-2023 / Date Published: 12-06-2025
Oyster mushrooms (Pleurotusspp.) are widely consumed in the world due to their delicious taste, high nutritional value and medicinal properties. For breeding program to develop new cultivars, besides phenotypic characteristics genetic characterization of the material is important since it helps breeders to choose suitable materials for hybridization. This study analyzed the genetic diversity of 10 oyster mushroom accessions using 20 RAPD markers and 17 ISSR markers to provide information for oyster mushroom breeding programs. The markers amplified a total of 560 loci with 552 polymorphic loci and 2,138 PCR product bands. When combining both RAPD and ISSR markers, the genetic similarity coefficient of 10 oyster accessions ranged from 0.459 to 0.755. At the average genetic similarity coefficient of 0.579, 10 oyster mushroom accessions were divided into 3 main groups. These results indicate that 10 collected oyster mushroom accessions had a high level of genetic diversity and can be used for new oyster mushrooms breeding program.
DOI: https://doi.org/10.31817/tckhnnvn.2023.21.12.
Genetic Diversity and Population Structure of Passion Fruit (Passiflora edulis Sim.) Accessions Revealed by SRAP and ISSR Markers

Tran Duc Trung, Nguyen Van Viet, Bui Quang Dang

Date Received: 14-10-2021 / Date Accepted: 01-03-2022
Identification of potential germplasms is the crucial step for selection and breeding program of passion fruit (Passiflora edulisSims.). In this study, genetic diversity and population structure of 31 exotic passion fruit accessions (including wild relative P. incarnata accessions) were assessed using sequence-related amplified polymorphism (SRAP) and inter simple sequence repeat (ISSR) markers. By primary screening for marker specificity and polymorphism, 8/15 SRAP markers and 10/18 ISSR were selected for passion fruit genotyping. The heterozygosity index H(0.43), polymorphic information content PIC(0.35), and discriminating power D(0.8) were recorded at a high level implying the significant genetic diversity of the analyzed passion fruit accessions. The Neighbor-Joining phylogenetic tree in combination with population structure model revealed by STRUCTURE discriminated and clustered the studied accessions into five groups which showed high accordance with the fruit color and the origin of the accessions. The analysis of melecular variance (AMOVA) also indicated the high rate of intraspecific and possibility interspecific hybridization among Passifloraspecies. These results provide essential genetic background facilitating efficient conservation and utilization of exotic passion fruit accessions in Vietnam.
Genetic Diversity of Hibiscus rosa-sinensisL. Accessions Collected in Hanoi and Hung Yen Province

Pham Thi Ngoc, Ngo Thi Hong Tuoi, Nguyen Thi Bich Hong, Doan Thu Thuy

Date Received: 05-12-2022 / Date Accepted: 18-04-2023 / Date Published: 12-06-2025
The study aims to evalute for morphological characteristics and genetic diversity of hibiscus. Twenty-four Hibiscus accessions collected in Hanoi and Hung Yen provinces (denoted as DB1 - DB24) were evaluated based on 12 morphological markers and 9 molecular markers (SSR and ISSR). The resutls showed that Hibiscus accessions exhibited significant variation in leaf morphology and flower colors, with 5 flower color groups, i.e white, pink, yellow, orange and red. The red color was predominant with 9 accessions. The genetic diversity analysis using 12 morphological characteristics grouped hibiscus accessions into 5 groups at similarity coefficient of 0.55 similarity, of which DB4 and DB20 were in each separate group. Nine molecular markers SSR and ISSR produced 33 allenswith average of 3.67 allens/markers and all were polymorphic. PIC values ranged from 0.05 to 0.47 in which HB15 marker had the highest PIC value. With the similarity coefficient at 0.64, 24 accessions were divided into 3 distinct clusters.
DOI: https://doi.org/10.31817/tckhnnvn.2023.21.4.
Assessement of Land Management and Land Use of Economic Organizations Which Were Assigned or Leased Land by the Government in Me Linh District, Ha Noi City

Do Thi Tam, Pham Minh Giap

Date Received: 18-03-2015 / Date Accepted: 09-06-2015 / Date Published: 13-06-2025
The study aims to assess land management and land useof economicorganizationswhich were assigned or leased land by the governmentin Me Linh. Thus, propose solutionsto enhanceland use efficiency, at the same time to limit the cases of breaking the Land law. The research used intergrated analysis and comparisonmethods. The result shows thatthere were 217economicorganizationswhich wereassigned or leased land with a total area of 927.50 ha in Me Linh District. 25 organizations used the land for improper purposes with an area of 14.27 ha; 9organizations used land for leasing to another organization with an area of 13.20 ha; 5 organizations had land in dispute with an area of 13.10 ha; 16 organizations used land as a join capital in business with an area of 34.74 ha and 13 organizations with 84.55 ha of land which have not put in to use yet. To remedy thesesituations, it is necessary to promulgate appropriate policies and laws, economical solutions, scientific solutions and management solutions.
DOI: https://doi.org/10.31817/tckhnnvn.2015.13.4.
Preliminary Evaluation of Potential Use of Simple Sequence Repeats (SSRs) in Studying Genetic Relationships among Isolates of Aspergillus spp.

Nguyen Khac Hai, Do Hai Quynh, Bui Thi Thanh, Nguyen Van Thuong, Nguyen Ngoc Hoa, Nguyen Ngoc Chinh, Dang Xuan Nghiem

Date Received: 01-10-2012 / Date Accepted: 12-12-2012
This study is aimed at exploring simple sequence repeats (SSRs) arrays and their polymorphism in Aspergillus oryzae, laying the foundations for the application of those sequences as molecular markers in investigation of genetic relationships among Aspergillus spp. isolates and in identification of a certain Aspergillus spp. strain. In silico data reveals that there is a total of 841 SSRs in the nuclear genome of Aspergillus oryzae. The density of those SSRs on 8 chromosomes ranges from 16 to 26 SSRs per Mb. The perfect SSRs sequences with 100% conserved repeats are in the majority, accounting for 44,6% of all 841 with the conservation bigger than 70%. The successful amplification of 17 SSR and iSSR loci revealed a high average number of alleles of 4,88 and a high genetic diversity with the average PIC value of 0,6 for each locus. Especially, there are specific patterns of allele number and length for each groups of the investigated moulds. This results confỉmed a high potential application of those SSR loci for detecting the presence of those moulds in human food and animal feed.

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