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• The Effects of Cell Density, Temperature and pH on Lactoferrin Productionfrom Recombinant Pichia PastorisKM71H-3 Strain

  Nguyen Thi Thuy, Trinh Thi Thu Thuy

  Date Received: 18-05-2020 / Date Accepted: 23-12-2020

   Abstract PDF (VIETNAMESE)

  Lactoferrin (LF) is a glycoprotein of the transferrin family whose general function is to transport iron. LF is capable of enhancing the immune system, antibacterial, antioxidant, anti-tumor, and antiviral activities. The gene encoding LF derived from bovine has been successfully expressed in recombinant P. pastoris KM71H-3 strain on 2xMMP medium. This study aims at investigating the effects of culture conditions including initial biomass cell density (equivalent to OD600 value from 0.5-2.0), temperature (20°C, 24°C,28°C), and initial pH of the medium (5.5-7.5). The LF expression level was evaluated by SDS – PAGE and Dot bloting methods. The SDS- PAGE andDot - blot results showed that LF was expressed in 2xMMP mediumwell with the optimal conditions were at 28°C, pH 6.0 and the initial biomass cell density (OD600) 0.5.