Date Received: 07-02-2025 / Date Published: 21-02-2025
Shiitake mushroom is a delicious and nutritious mushroom because it contains diverse nutritional components and valuable medicinal active ingredients. Currently, the source of spawn for cutivation of shiitake mushroom in Vietnam is mainly imported from China, Korea or Japan. Collecting and researching native shiitake mushroom genetic resources in Vietnam is so meaningful for the conservation genetic resources. The LeCT mushroom strain is a native shiitake strain which collected in Cat Tien National Park (Vietnam)... The purpose is to find the most suitable conditions for the mycelial growth of shiitake strain LeCT. Mycelial of strain were cultured in different temperature levels, media pH values, carbon sources, mineral salt sources and spawning substrates. The findings show that the most optimal temperature for mycelial growth is 25°C ± 2, the culture medium with pH value of 6 gives the best mycelial growth results. Glucose and MgSO4.7H2O are the best sources of carbon and mineral salts for mycelial growth. Substrate includes 77% boiled rice, 20% corn cob, 2% barley bran and 1% CaCO3 performs the best mycelial growth results.
