Search

Search Results

• Isolation and Identification Biochemical Characteristics of Porcine Circovirus Type 2 (PCV2) from pigs in Some Northern Provinces of Vietnam

  Huynh Thi My Le, Nguyen Van Giap

  Date Received: 15-04-2013 / Date Accepted: 15-06-2013 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  The aim of this study was to isolate and identify biochemichal characteristics of PCV2formpigs with PCVAD in 4 nothern provinces of Vietnam (Hanoi, Hoa Binh, Bac Giang and Hai Duong). Isolation of PCV2 would be a conclusive evidence to confirm the circulation of PCV2 and PCVAD in pigs of Vietnam. 8 of PCV2 were isolated from 38 samples collected from pigs with suspected PCVAD. PCV2 isolates were PCV2b genotype. The result of identification biochemical characteristics of isolated PCV2 shown that PCV2 could multiply in PK15 cell culture from the first or second passage, but no CPE was observed. TCID50of all ranging from 102,7to104,2 /ml.

  DOI: https://doi.org/10.31817/tckhnnvn.2013.11.3.

• A Molecular Based Study on The Spatio-Temporal Dynamics of Porcine Circovirus Genotype 2d (PCV2d) Circulating in Vietnam

  Pham Hong Quan, Pham Cong Hoat, Huynh Thi My Le

  Date Received: 04-05-2017 / Date Accepted: 09-06-2017

   Abstract PDF (VIETNAMESE)

  This study aimed at providing an up-to-date information on the molecular epidemiology of porcine circovirus type 2 (PCV2) circulating in Vietnam with a focus on the spatio-temporal dynamics of a newly defined PCV2d genotype. The results revealed a presence of multiple genotypes/clusters of PCV2, such as 1A/1B, circulating recombinant form (CRF) of PCV2b, and PCV2d. In northern, central and southern Vietnam, a co-circulation of multiple genetic clusters of PCV2 was observed in each sampling location at the same time period. All field strains of PCV2d circulating in Vietnam from 2004- 2017 were predicted to have the most common recent ancestor originated from China, however, they located at different branches of the PCV2d- phylogenetic tree.

• Clinical Signs and Pathological Lesions in Pigs with PCV2 Associated - Postweaning Multisystemic Wasting Syndrome in Some Northern Provinces of Vietnam

  Huynh Thi My Le, Nguyen Van Giap, Dang Huu Anh, Nguyen Ba Tiep

  Date Received: 23-08-2012 / Date Accepted: 16-12-2012 / Date Published: 02-07-2025

   Abstract PDF (VIETNAMESE)

  The aim of this study was to determine the main clinical signs and pathological  lesions of pigs with PCV2 - associated PMWS. Thirty nine postweaning pigs with suspected PCV2-associated PMWS were collected from 16 farms in four Nothern provinces of Vietnam (Hanoi, Hoa Binh, Bac Giang and Hai Duong). Clinical signs included wasting with weight loss, ill-thrift and rough hair coat (100%), respiratory clinical signs (89.74%), jaundice (15.38%), diarrhea (23.08%), neurologic signs (10.26%) and dermatitis (20.51%). Gross lesion finding included body prominent backbone and swollen, hemorrhagic lympho nodes (ranging from 94.87% to 100%). Gross lesions could also be found in the tonsil (53.85%), lung (92.31%), liver (41.02%), kidney (30.77%), intestines (28.21%) and spleen (17.95%), and arthritis (7.69%). Microscopic lesions consisted of vasculitis and granulomatous inflammation with the forming of multinucleated giant cells in the lung, superficial inguinal lymph nodes, kidney and liver. Using nested PCR method, PCV2b antigen were positive in 94.87% of samples from suspected pigs.

  DOI: https://doi.org/10.31817/tckhnnvn.2012.10.7.