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• Vegetative Propagation of Phalaenopsis Sogo Yukidian

  Nguyen Thi Son, Nguyen Quang Thach, Nguyen Thi Ly Anh, Hoang Thi Nga, Hoang Thi Anh Nguyet

  Date Received: 16-10-2014 / Date Accepted: 24-11-2014 / Date Published: 06-08-2025

   Abstract PDF (VIETNAMESE)

  Phalaenopsis Sogo Yukidian is a beautiful, lovely and high value orchid in the world and also in Vietnam. An in vitro culture protocol for rapid propagation was established to multiply this valuable flower. Nodal buds of inflorescence of Phalaenopsis Sogo Yukidian were used as initial materials. Optimal sterilization treatment was obtained with CaOCl2 15% for 7 min and then with Presept (Johnson and Johnson) 1% for 3 min. The optimal medium for protocorm regeneration was MS + 2.0 mg/l BA + 6g/l agar. The optimal medium for shoot induction was MS + 0.5mg/l BA + 0.5mg/l α NAA + 6g/l agar. Shoots grew fastest on MS medium supplemented with 10% coconut water. MS medium supplemented with 10% coconut water + 3.0mg/l BA + 6g/l agar was the best medium for shoot multiplication with the multiplication rate of 4.20 times after 6 weeks. MS medium supplemented with 10% coconut water + 0.5 g/l activate charcoal + 6g/l agar was the suitable for rooting.

  DOI: https://doi.org/10.31817/tckhnnvn.2014.12.8.