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• Genetic Diversity in Common Bean Accessions Evaluated by Means of Morpho-Agronomical and SSR Data

  Pham Thi Ngoc, Nguyen Quoc Trung, Vu Van Liet

  Date Received: 12-05-2016 / Date Accepted: 20-12-2016 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  The knowledge and understanding of genetic variability of common bean (Phaseolus vulgarisL.) germplasm is important for utilization and conservation. The Oobjective of this study was to analyzesis ofgenetic diversity of 60 accessions that collected in Vietnam and from other countries. Results based on mMorphological markers showed that 60 germplasms accessions werearevery diverse and they were dievided into 7 groups with genetic similarity index of 0.17. Using SSR markers to evaluate genetic diversity,Fifteen15SSR markersamong 20 used wereshowed polymorphismcwith total of 69 alleleswith and an average of 4.6 alleles per locus. BM152 marker had highest PIC value (0.73). Four major clusters were grouped for 60 accessions with genetic similarity at 0.69. The,dendrogram showing the genetic relationships was constructed by the unweighted pairedgroup method with arithmetic average (UPGMA) using the software NTSYS-pc 2.01. The results of this study indicatedthat SSR analysis could be successfully used for the estimation of genetic diversity among common bean accessions. The diversity of common beanThatare of the highest usefulnessfor breeding purposes.

  DOI: https://doi.org/10.31817/tckhnnvn.2016.14.12.

• IN VITRO PROPAGATION OF COMMON BEAN (Phaseolus vulgaris L.)

  Ninh Thi Thao, Nguyen Thi Phuong Thao, Fathi Hassan, Hans Jörg Jacobsen

  Date Received: 06-07-2013 / Date Accepted: 25-08-2013 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

   The study was conducted to establish the protocol for in vitro regeneration of common bean (Phaseolus vulgaris L.). Cotyledonary nodes excised from 7-day in vitro seedlings were used as primary explants. The optimum treatment for shoot induction was MS salts and B5 vitamins (MSB5) medium supplemented with 11.1µM BAP and 0.5µM α-NAA, where 86.7% of cotyledonary nodes induced shoots with an average of 3.1 shoots/explant after 4 weeks of culture. Elongated, well developed individual shoots with 3-6 leaves were transferred to rooting medium containing 4.9µM of IBA. The average rooting frequency was 53.3% with a mean of 1.7 roots/shoot after 4 weeks of culture. 80% rooted plantlets survived during acclimatization period in greenhouse.

  DOI: https://doi.org/10.31817/tckhnnvn.2013.11.6.