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• In vitro propagation of papaya Carica papapaya L.

  Do Van Nam, Nguyen Thi Thuy, Nguyen Thi Bich Hong, Pham Thi Ngoc, Nguyen Van Hoan, Nguyen Thi Phuong Thao

  Date Received: 04-06-2013 / Date Accepted: 21-09-2013 / Date Published: 13-06-2025

   Abstract PDF (VIETNAMESE)

  This study was conducted to establish the protocol for in vitro propagation of papaya using stem segments. Results showed that double sterilization of explants with HgCl2 0.1% first  for 10 minutes and follwed by 5 minutes was most appropriate. MS medium containing 1 mg/l α-NAA+ 0,5 mg/l BA was optimal for callus induction (90.74%) of young stem slices after 4 weeks of culture. The highest shoot multiplication rate (3.17) was obtained on MS medium supplemented with 1.0 mg/l BA and 0.1 mg/l α- NAA after 4 weeks. Root induction medium was ½ MS containing 1.0 mg/l activated charcoal and 1.5 mg/l IBA. The average rooting percentage  was 50% within 4 weeks.

  DOI: https://doi.org/10.31817/tckhnnvn.2013.11.6.