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• Transformation of Sugarcane (Saccharum officinarum L.) with Insect Resistance Genes cry1ab, cry1b-cry1ab

  Phan Tuong Loc, Hoang Van Duong, Mai Trường, Le Tan Duc, Tran Thi Ngoc Ha, Van Dac Thanh, Pham Duc Tri, Nguyen Thi Thanh, Nguyen Huu Ho

  Date Received: 25-07-2014 / Date Accepted: 14-10-2014 / Date Published: 06-08-2025

   Abstract PDF (VIETNAMESE)

  The sugarcane variety VN84-4137 that was successfully confirmed with preliminary transformation experiments was further transformed with  Bt genes including a single synthetic gene cry1Ab and a hybrid synthetic gene cry1B-cry1Ab. After infection and co-culture with Agrobacterium, sugarcane calli were selected on callus induction medium with 3 mg/l phosphinothricin (PPT). Resistant calli were transferred to selective regeneration medium for shoot induction. Three cry1Ab lines and one cry1B-cry1Ab transgenic line were identified by PCR and Southern blot. Protein expression of Cry1Ab and Cry1B-Cry1Ab assayed by quick test kit gave positive results. Transgenic plants exhibited a normal phenotype.

  DOI: https://doi.org/10.31817/tckhnnvn.2014.12.7.